Mouse Tissue Factor Pathway Inhibitor 2 ELISA Kit | TFPI2 elisa kit

Mouse Tissue Factor Pathway Inhibitor 2 ELISA Kit

Cat. Num. AAA764995

• Gene Names: TFPI2; PP5; REF1; TFPI-2
• Reactivity: Mouse
• Synonyms: Tissue Factor Pathway Inhibitor 2; Mouse Tissue Factor Pathway Inhibitor 2 ELISA Kit; TFPI2/PP5/REF-1/Placental protein 5/PP5FLJ21164/TFPI-2REF1/tissue factor pathway inhibitor 2; TFPI2 elisa kit

• Reactivity: Mouse
• Sequence Length: 235

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TFPI2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse TFPI2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse TFPI2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TFPI2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse TFPI2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5730091
• NCBI GeneID: 7980
• NCBI Accession #: NP_006519.1
• NCBI GenBank Nucleotide #: NM_006528.3
• Molecular Weight: 25,796 Da
• NCBI Official Full Name: tissue factor pathway inhibitor 2 isoform 1
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor 2
• NCBI Official Symbol: TFPI2
• NCBI Official Synonym Symbols: PP5; REF1; TFPI-2
• NCBI Protein Information: tissue factor pathway inhibitor 2
• UniProt Protein Name: Tissue factor pathway inhibitor 2
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI2
• UniProt Synonym Gene Names: TFPI-2; PP5
• UniProt Entry Name: TFPI2_HUMAN

NCBI Description

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: nucleus; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; serine-type endopeptidase inhibitor activity

Biological Process: blood coagulation