Chicken Tissue Factor Pathway Inhibitor 2 ELISA Kit | TFPI2 elisa kit

Chicken Tissue Factor Pathway Inhibitor 2 ELISA Kit

Cat. Num. AAA739481

• Gene Names: TFPI2; PP5; REF1; TFPI-2
• Reactivity: Chicken
• Synonyms: Tissue Factor Pathway Inhibitor 2; Chicken Tissue Factor Pathway Inhibitor 2 ELISA Kit; TFPI2 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This TFPI2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken TFPI2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFPI2 elisa kit

Principle of the Assay: TFPI2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TFPI2. Standards or samples are then added to the microtiter plate wells and TFPI2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TFPI2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TFPI2 are added to each well to "sandwich" the TFPI2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TFPI2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFPI2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TFPI2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 5730091
• NCBI GeneID: 7980
• NCBI Accession #: NP_006519.1
• NCBI GenBank Nucleotide #: NM_006528.2
• UniProt Accession #: P48307; Q66ME8; Q9UC86; Q8NAK6; Q8NE89; Q96QP2
• Molecular Weight: 26,934 Da
• NCBI Official Full Name: tissue factor pathway inhibitor 2
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor 2
• NCBI Official Symbol: TFPI2
• NCBI Official Synonym Symbols: PP5; REF1; TFPI-2
• NCBI Protein Information: tissue factor pathway inhibitor 2; placental protein 5
• UniProt Protein Name: Tissue factor pathway inhibitor 2
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI2
• UniProt Synonym Gene Names: TFPI-2; PP5
• UniProt Entry Name: TFPI2_HUMAN

NCBI Description

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: proteinaceous extracellular matrix; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; extracellular matrix structural constituent

Biological Process: blood coagulation