Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DIS3-like exonuclease 1 (DIS3L) Standard Curve.)
Human DIS3-Like Exonuclease 1 (DIS3L) ELISA Kit | DIS3L elisa kit
Human DIS3-Like Exonuclease 1 (DIS3L) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DIS3-like exonuclease 1 (DIS3L) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DIS3-like exonuclease 1 (DIS3L) Standard Curve.)
Background: Dis3L is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the cytoplasm of eukaryotic cells. DIS3L belongs to the ribonuclease II (RNB) family. Ribonuclease II (RNase II) is a major exoribonuclease that intervenes in all of these fundamental processes; it can act independently or as a component of the exosome, an essential RNA-degrading multiprotein complex. RNase II-like enzymes are found in all three kingdoms of life, but there are no structural data for any of the proteins of this family. RNase R is a close homolog of RNase II, but it can, unlike RNase II, degrade RNA with secondary structures without help of accessory factors.
NCBI and Uniprot Product Information
NCBI Description
The cytoplasmic RNA exosome complex degrades unstable mRNAs and is involved in the regular turnover of other mRNAs. The protein encoded by this gene contains 3'-5' exoribonuclease activity and is a catalytic component of this complex. [provided by RefSeq, May 2016]
Uniprot Description
Function: Putative cytoplasm-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. Ref.6 Ref.7
Cofactor: Magnesium. Ref.6 Ref.7
Subunit structure: Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms.
Subcellular location: Cytoplasm Ref.6 Ref.7.
Sequence similarities: Belongs to the RNR ribonuclease family.
Sequence caution: The sequence BAB85541.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Research Articles on DIS3L
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Product Notes
The Human DIS3L dis3l (Catalog #AAA9715711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715711 ELISA Kit recognizes Human DIS3L. It is sometimes possible for the material contained within the vial of "DIS3-Like Exonuclease 1 (DIS3L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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