Typical Testing Data/Standard Curve
Bovine Oviduct-specific glycoprotein (OVGP1) ELISA Kit | OVGP1 elisa kit
Bovine Oviduct-specific glycoprotein (OVGP1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||OVGP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OVGP1 antibody and an OVGP1-HRP conjugate. The assay sample and buffer are incubated together with OVGP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OVGP1 concentration since OVGP1 from samples and OVGP1-HRP conjugate compete for the anti-OVGP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OVGP1 from the sample, fewer sites are left to bind OVGP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OVGP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a large, carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites located within threonine, serine, and proline-rich tandem repeats. The gene is similar to members of the mucin and the glycosyl hydrolase 18 gene families. Regulation of expression may be estrogen-dependent. Gene expression and protein secretion occur during late follicular development through early cleavage-stage embryonic development. The protein is secreted from non-ciliated oviductal epithelial cells and associates with ovulated oocytes, blastomeres, and spermatozoan acrosomal regions. [provided by RefSeq, Jul 2008]
Uniprot Description
OVGP1: Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development. Belongs to the glycosyl hydrolase 18 family.
Chromosomal Location of Human Ortholog: 1p13
Cellular Component: transport vesicle; extracellular region; cytosol
Molecular Function: chitin binding; chitinase activity
Biological Process: binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; chitin catabolic process; female pregnancy; multicellular organism reproduction
Research Articles on OVGP1
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Product Notes
The Bovine OVGP1 ovgp1 (Catalog #AAA7252325) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7252325 ELISA Kit recognizes Bovine OVGP1. It is sometimes possible for the material contained within the vial of "Oviduct-specific glycoprotein (OVGP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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