Typical Testing Data/Standard Curve (for reference only)
Human Coiled Coil Domain Containing Protein 99 (CCDC99) ELISA Kit | CCDC99 elisa kit
Human Coiled Coil Domain Containing Protein 99 (CCDC99) ELISA Kit
Reactivity
Human
Synonyms
Coiled Coil Domain Containing Protein 99 (CCDC99); Human Coiled Coil Domain Containing Protein 99 (CCDC99) ELISA Kit; Spindly; Arsenite-related gene 1 protein; Rhabdomyosarcoma antigen MU-RMS-40.4A; Spindle apparatus coiled-coil domain-containing protein 1; CCDC99 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CC16. No significant cross-reactivity or interference between CC16 and analogues was observed.
Sequence Length
607
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Lung Lavage Fluid, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
78-5,000pg/mL
Sensitivity
30pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CC16 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CC16 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCDC99 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CC16 in mouse serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CC16. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CC16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CC16. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CC16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for CCDC99 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
70,138 Da
NCBI Official Full Name
Coiled-coil domain-containing protein 99, partial
UniProt Protein Name
Protein Spindly
UniProt Gene Name
SPDL1
UniProt Synonym Gene Names
CCDC99UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03041
Uniprot Description
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) ().
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Product Notes
The Human CCDC99 spdl1 (Catalog #AAA2105033) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2105033 ELISA Kit recognizes Human CCDC99. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the CCDC99 spdl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coiled Coil Domain Containing Protein 99 (CCDC99), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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