TEN1 recombinant protein

TEN1, 1-123aa, Human, His tag, E Coli

Cat. Num. AAA205419

• Gene Names: TEN1; C17orf106
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TEN1; 1-123aa; Human; His tag; E Coli; CST complex subunit TEN1; TEN1 telomerase capping complex subunit homolog; C17orf106; TEN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 7.5) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMLPKPG TYYLPWEVSA GQVPDGSTLR TFGRLCLYDM IQSRVTLMAQ HGSDQHQVLV CTKLVEPFHA QVGSLYIVLG ELQHQQDRGS VVKARVLTCV EGMNLPLLEQ AIREQRLYKQ ERGGSQ
• Sequence Length: 123

• Applicable Applications for TEN1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TEN1 recombinant protein

TEN1 is component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Recombinant human TEN1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TEN1 recombinant protein

Epigenomics

References

Miyake Y., et al. (2009) Mol. Cell 36:193-206.

NCBI and Uniprot Product Information

• NCBI GI #: 319918878
• NCBI GeneID: 100134934
• NCBI Accession #: NP_001106795
• NCBI GenBank Nucleotide #: NM_001113324.2
• Molecular Weight: 16.2kDa (146aa), confirmed by MALDI-TOF
• NCBI Official Full Name: CST complex subunit TEN1
• NCBI Official Synonym Full Names: TEN1, CST complex subunit
• NCBI Official Symbol: TEN1
• NCBI Official Synonym Symbols: C17orf106
• NCBI Protein Information: CST complex subunit TEN1
• UniProt Protein Name: CST complex subunit TEN1
• Protein Family: Telomere length regulation protein
• UniProt Gene Name: TEN1
• UniProt Synonym Gene Names: C17orf106

NCBI Description

C17ORF106, or TEN1, appears to function in a telomere-associated complex with STN1 (OBFC1; MIM 613128) and CTC1 (C17ORF68; MIM 613129) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]

Uniprot Description

Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22763445). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097).