Testing Data
Mouse anti-Mouse Nonstructural Protein 9 (nsp9) Monoclonal Antibody | anti-nsp9 antibody
Anti-Mouse Hepatitis Virus (Strain A59) Nonstructural Protein 9 (nsp9) Antibody
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Protein A Purified
Synonyms
Nonstructural Protein 9 (nsp9); Monoclonal Antibody; Anti-Mouse Hepatitis Virus (Strain A59) Nonstructural Protein 9 (nsp9) Antibody; MHV-A59; anti-nsp9 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Specificity
This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.
Purity/Purification
Protein A Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
335
Applicable Applications for anti-nsp9 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
Immunogen
This antibody was produced in mice by repeated immunizations with E.coli derived fulllength MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for anti-nsp9 antibody
The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nonstructural protein NSP9
Similar Products
Product Notes
The nsp9 (Catalog #AAA4157741) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse Hepatitis Virus (Strain A59) Nonstructural Protein 9 (nsp9) Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nonstructural Protein 9 (nsp9) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. Researchers should empirically determine the suitability of the nsp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nonstructural Protein 9 (nsp9), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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