Mouse anti-Human, Mouse UHRF1 Monoclonal Antibody | anti-UHRF1 antibody

UHRF1 (N-terminus) Monoclonal Antibody

Cat. Num. AAA3014252

• Gene Names: UHRF1; Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UHRF1; Monoclonal Antibody; UHRF1 (N-terminus) Monoclonal Antibody; Np95; hNP95; ICBP90; RNF106; FLJ21925; MGC138707; E3 ubiquitin-protein ligase UHRF1; Nuclear protein 95; HuNp95; hNp95; RING finger protein 106; anti-UHRF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: This antibody detects endogenous levels of UHRF1 (N-terminus) and does not cross-react with related proteins
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 793

• Applicable Applications for anti-UHRF1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:50-200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human UHRF1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot detection of UHRF1 antibody in Mouse heart, 293T, HelaNE&Hela cell lysates using UHRF1 antibody (1:1000 diluted).)

Immunocytochemistry (ICC)

(Immunocytochemistry of HeLa cells using anti- UHRF1 (N-terminus) antibody diluted 1:200.)

Related Product Information for anti-UHRF1 antibody

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 115430235
• NCBI GeneID: 29128
• NCBI Accession #: NP_001041666.1
• NCBI GenBank Nucleotide #: NP_001041666.1
• UniProt Accession #: Q96T88
• Molecular Weight: Predicted Band Size: 90KDa ; Observed Band Size: 90KDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase UHRF1 isoform 1
• NCBI Official Synonym Full Names: ubiquitin like with PHD and ring finger domains 1
• NCBI Official Symbol: UHRF1
• NCBI Official Synonym Symbols: Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
• NCBI Protein Information: E3 ubiquitin-protein ligase UHRF1
• UniProt Protein Name: E3 ubiquitin-protein ligase UHRF1
• Protein Family: UHRF1-binding protein
• UniProt Gene Name: UHRF1
• UniProt Synonym Gene Names: ICBP90; NP95; RNF106; HuNp95; hNp95; hUHRF1
• UniProt Entry Name: UHRF1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]