Typical Testing Data/Standard Curve (for reference only)
Mouse Transcription factor 7-like 2 ELISA Kit | Tcf7l2 elisa kit
Mouse Transcription factor 7-like 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TCF7L2: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. Belongs to the TCF/LEF family. 10 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding; Cell development/differentiation; Cell cycle regulation; Apoptosis
Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex
Molecular Function: beta-catenin binding; chromatin binding; DNA binding; gamma-catenin binding; nuclear hormone receptor binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: blood vessel development; bone mineralization; cell cycle arrest; cell glucose homeostasis; cell proliferation; cellular response to starvation; embryonic digestive tract morphogenesis; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis; fat cell differentiation; glucose homeostasis; glucose metabolic process; glycogen metabolic process; maintenance of DNA repeat elements; multicellular organism growth; myoblast cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of fat cell differentiation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of organ growth; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube development; odontogenesis of dentine-containing teeth; oligodendrocyte development; pituitary gland development; positive regulation of apoptosis; positive regulation of epithelial cell proliferation; positive regulation of gluconeogenesis; positive regulation of insulin secretion; positive regulation of lipid biosynthetic process; positive regulation of protein binding; positive regulation of protein export from nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; post-embryonic development; regulation of hormone metabolic process; regulation of myelination; regulation of oligodendrocyte differentiation; regulation of skeletal muscle development; regulation of smooth muscle cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to glucose stimulus; secretory granule localization; skin development; somatic stem cell maintenance; transcription, DNA-dependent; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Research Articles on Tcf7l2
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Product Notes
The Mouse Tcf7l2 tcf7l2 (Catalog #AAA2887486) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2887486 ELISA Kit recognizes Mouse Tcf7l2. It is sometimes possible for the material contained within the vial of "Transcription factor 7-like 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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