TAOK2 / Serine/Threonine-Protein Kinase TAO2 Recombinant Protein | TAOK2 recombinant protein

Human TAOK2 / Serine/Threonine-Protein Kinase TAO2 Recombinant Protein

• Gene Names: TAOK2; PSK; PSK1; TAO1; TAO2; MAP3K17; PSK1-BETA
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: TAOK2 / Serine/Threonine-Protein Kinase TAO2; Human TAOK2 / Serine/Threonine-Protein Kinase TAO2 Recombinant Protein; Serine/threonine-protein kinase TAO2; Kinase from chicken homolog C; hKFC-C; Prostate-derived sterile 20-like kinase 1; PSK-1; Thousand and one amino acid protein kinase 2; KIAA0881; MAP3K17; PSK; PSK1; UNQ2971/PRO7431; TAOK2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 1235

• Applicable Applications for TAOK2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with MAP2K3 and MAP2K6 (By similarity). Self-associates. Interacts with tubulins through the C-terminal domain. Interacts with MAP3K7 and interfers with MAP3K7-binding to CHUK and thus prevents NF-kappa-B activation. Isoform 2 interacts with PCDH8; this complex may also include CDH2.
• Entry Function: Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 116242813
• NCBI GeneID: 9344
• UniProt Accession #: Q9UL54
• NCBI Official Full Name: Serine/threonine-protein kinase TAO2
• NCBI Official Synonym Full Names: TAO kinase 2
• NCBI Official Symbol: TAOK2
• NCBI Official Synonym Symbols: PSK; PSK1; TAO1; TAO2; MAP3K17; PSK1-BETA
• NCBI Protein Information: serine/threonine-protein kinase TAO2
• UniProt Protein Name: Serine/threonine-protein kinase TAO2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: TAOK2
• UniProt Synonym Gene Names: KIAA0881; MAP3K17; PSK; PSK1; hKFC-C; PSK-1; PSK1; Prostate-derived STE20-like kinase 1
• UniProt Entry Name: TAOK2_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TAO2: a serine/threonine-protein kinase of the STE20 family. Activates the p38 MAPK signaling pathway through the specific activation of the upstream kinases MKK3 and MKK6. A potential multi-pass membrane protein. Activated in the DNA damage response and regulates the G2/M transition DNA damage checkpoint. Interacts with tubulins through the C-terminal domain. Involved in apoptotic processes such as membrane blebbing and apoptotic body formation. Prevents TAK1-mediated activation of IKK-alpha, and thus NF-kappa-B activation. May play a role in the osmotic stress-JNK1 pathway. An autism spectrum disorder susceptibility gene. Affects basal dendrite formation and axonal projections in cortical pyramidal neurons. Interacts with NRP1 (neuropilin 1), a receptor for the secreted guidance protein semaphorin 3A (Sema3A). Influences the formation of basal dendrites through the activation of JNK. Three isoforms of the human protein are produced by alternative splicing. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus.

Protein type: Kinase, protein; Membrane protein, integral; Apoptosis; Protein kinase, STE; Membrane protein, multi-pass; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; TAO subfamily

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytoplasmic vesicle membrane; cytoskeleton; cytoplasmic membrane-bound vesicle; dendrite; cytoplasm; nucleolus; integral to membrane; nucleus; receptor complex

Molecular Function: protein serine/threonine kinase activity; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; ATP binding

Biological Process: focal adhesion formation; cell migration; activation of MAPKK activity; apoptosis; stress-activated MAPK cascade; positive regulation of JNK cascade; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; regulation of cell shape; response to stress; regulation of cell growth; G2/M transition DNA damage checkpoint; actin cytoskeleton organization and biogenesis; protein targeting to membrane; response to DNA damage stimulus