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Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Mouse anti-Rat Vitamin D Binding Protein (DBP) Monoclonal Antibody | anti-DBP antibody
Monoclonal Antibody to Vitamin D Binding Protein (DBP)
Gene Names
Gc; DBP; VDB; Vdbp; DBP02
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Vitamin D Binding Protein (DBP); Monoclonal Antibody; Monoclonal Antibody to Vitamin D Binding Protein (DBP); GC; VDBG; VDBP; Group-Specific Component; Gc-globulin; anti-DBP antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C4
Specificity
The antibody is a mouse monoclonal antibody raised against DBP. It has been selected for its ability to recognize DBP in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DBP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Immunogen
Recombinant Vitamin D Binding Protein (DBP) (Leu209~Arg394)
Cross Reactivity
Rat
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Label
None
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2059396
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DBP, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant DBP, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
53,544 Da
NCBI Official Full Name
Vitamin D-binding protein
NCBI Official Synonym Full Names
GC, vitamin D binding protein
NCBI Official Symbol
Gc
NCBI Official Synonym Symbols
DBP; VDB; Vdbp; DBP02
NCBI Protein Information
vitamin D-binding protein
UniProt Protein Name
Vitamin D-binding protein
Protein Family
UniProt Gene Name
Gc
UniProt Synonym Gene Names
Dbp; DBP; VDB
NCBI Description
transports vitamin D and vitamin D metabolites [RGD, Feb 2006]
Uniprot Description
Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Research Articles on DBP
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Product Notes
The DBP gc (Catalog #AAA2085911) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Vitamin D Binding Protein (DBP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vitamin D Binding Protein (DBP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the DBP gc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vitamin D Binding Protein (DBP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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