TGFB2 sirna

TGFB2 siRNA (Rat)

Cat. Num. AAA8225519

• Gene Names: Tgfb2; TGF-B2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TGFB2; TGFB2 siRNA (Rat); Transforming growth factor beta-2; TGF-beta-2; TGFB2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TGFB2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 442

• Applicable Applications for TGFB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TGFB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TGFB2 sirna

siRNA to inhibit TGFB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13592109
• NCBI GeneID: 81809
• NCBI Accession #: NP_112393.1
• NCBI GenBank Nucleotide #: NM_031131.1
• UniProt Accession #: Q07257; Q63574; Q9QW26; Q9R281; Q9R298; Q9R2B8; Q9WUQ8
• Molecular Weight: 47,728 Da
• NCBI Official Full Name: transforming growth factor beta-2
• NCBI Official Synonym Full Names: transforming growth factor, beta 2
• NCBI Official Symbol: Tgfb2
• NCBI Official Synonym Symbols: TGF-B2
• NCBI Protein Information: transforming growth factor beta-2
• UniProt Protein Name: Transforming growth factor beta-2
• Protein Family: Transforming growth factor
• UniProt Gene Name: Tgfb2
• UniProt Synonym Gene Names: TGF-beta-2; LAP
• UniProt Entry Name: TGFB2_RAT

NCBI Description

binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]

Uniprot Description

TGFB2: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN. Belongs to the TGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: axon; basement membrane; cell soma; cell surface; cytoplasm; endosome; extracellular matrix; extracellular region; extracellular space; secretory granule; trans-Golgi network

Molecular Function: beta-amyloid binding; cytokine activity; protein heterodimerization activity; protein homodimerization activity; protein N-terminus binding; punt binding; receptor binding; receptor signaling protein serine/threonine kinase activity; transforming growth factor beta receptor binding

Biological Process: activation of protein kinase activity; aging; axon guidance; blood vessel development; blood vessel remodeling; cardiac muscle cell proliferation; cardioblast differentiation; cartilage condensation; cell cycle arrest; cell death; cell development; cell migration; cell morphogenesis; collagen fibril organization; dopamine biosynthetic process; embryonic gut development; embryonic limb morphogenesis; embryonic neurocranium morphogenesis; epithelial to mesenchymal transition; extracellular matrix organization and biogenesis; eye development; female pregnancy; glial cell migration; gut development; hair follicle development; hair follicle morphogenesis; heart development; heart morphogenesis; hemopoiesis; hindbrain development; inner ear development; intercellular junction assembly and maintenance; kidney development; lung development; male gonad development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of immune response; negative regulation of Ras protein signal transduction; negative regulation of release of sequestered calcium ion into cytosol; neural tube closure; neuron development; neuron fate commitment; neutrophil chemotaxis; palate development; pancreas development; positive regulation of apoptosis; positive regulation of cardioblast differentiation; positive regulation of catagen; positive regulation of cell adhesion mediated by integrin; positive regulation of cell cycle; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of heart contraction; positive regulation of immune response; positive regulation of integrin biosynthetic process; positive regulation of neuron apoptosis; positive regulation of ossification; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein secretion; positive regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of apoptosis; regulation of cell growth; regulation of cell proliferation; regulation of MAPKKK cascade; regulation of transforming growth factor-beta2 production; response to cold; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to estrogen stimulus; response to hypoxia; response to organic nitrogen; response to progesterone stimulus; response to radiation; response to retinoic acid; response to vitamin D; response to wounding; salivary gland morphogenesis; skeletal development; skeletal muscle development; SMAD protein nuclear translocation; somatic stem cell division; thyroid gland development; transforming growth factor beta receptor signaling pathway; uterus development; wound healing