Typical Testing Data/Standard Curve (for reference only)
Rat transforming growth factor, beta 2 ELISA Kit | TGFB2 elisa kit
Rat Transforming Growth factor beta2, TGF-beta2 ELISA Kit
Gene Names
Tgfb2; TGF-B2
Reactivity
Rat
Synonyms
transforming growth factor; beta 2; Rat Transforming Growth factor beta2; TGF-beta2 ELISA Kit; TGF-beta2 ELISA kit; MGC116892; TGF-beta2; ; TGFB2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat TGFB2. No significant cross-reactivity or interference between Rat TGFB2 and analogues was observed.
Sequence Length
442
Samples
Serum, plasma and tissue homogenates
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TGFB2 elisa kit
Introduction: Transforming growth factor beta (TGF-beta) is a protein that comes in three isoforms called TGF-beta1, TGF-beta2 and TGF-beta3; it was also the original name for the founding member of this family that is now called TGF-beta1. The TGF-beta family is part of a superfamily of proteins known as the transforming growth factor beta superfamily, which includes inhibins, activin, anti-müllerian hormone, bone morphogenetic protein, decapentaplegic and Vg-1. TGF beta controls proliferation, differentiation, and other functions in most cell types. It can also act as a negative autocrine growth factor. TGF-beta2 is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development (alternative names: Glioblastoma-derived T-cell suppressor factor, G-TSF, BSC-1 cell growth inhibitor, Polyergin, Cetermin). It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. There are two named isoforms of this protein, created by alternative splicing of the same gene.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-beta2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-beta2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,534 Da
NCBI Official Full Name
transforming growth factor beta-2
NCBI Official Synonym Full Names
transforming growth factor, beta 2
NCBI Official Symbol
Tgfb2
NCBI Official Synonym Symbols
TGF-B2
NCBI Protein Information
transforming growth factor beta-2; TGF-beta-2; TGF beta 2 protein
UniProt Protein Name
Transforming growth factor beta-2
Protein Family
UniProt Gene Name
Tgfb2
UniProt Synonym Gene Names
TGF-beta-2; LAP
UniProt Entry Name
TGFB2_RAT
NCBI Description
binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]
Uniprot Description
Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Research Articles on TGFB2
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Product Notes
The Rat TGFB2 tgfb2 (Catalog #AAA702095) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702095 ELISA Kit recognizes Rat TGFB2. It is sometimes possible for the material contained within the vial of "transforming growth factor, beta 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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