TCEB2 sirna

TCEB2 siRNA (Rat)

Cat. Num. AAA8212140

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TCEB2; TCEB2 siRNA (Rat); Transcription elongation factor B polypeptide 2; Elongin 18 kDa subunit; Elongin-B; EloB; RNA polymerase II transcription factor SIII subunit B; SIII p18; TCEB2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TCEB2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 118

• Applicable Applications for TCEB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TCEB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TCEB2 sirna

siRNA to inhibit TCEB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13592105
• NCBI GeneID: 81807
• NCBI Accession #: NP_112391.1
• NCBI GenBank Nucleotide #: NM_031129.3
• UniProt Accession #: P62870; Q63529; Q80W20
• Molecular Weight: 13,170 Da
• NCBI Official Full Name: transcription elongation factor B polypeptide 2
• NCBI Official Synonym Full Names: transcription elongation factor B (SIII), polypeptide 2
• NCBI Official Symbol: Tceb2
• NCBI Protein Information: transcription elongation factor B polypeptide 2
• UniProt Protein Name: Transcription elongation factor B polypeptide 2
• Protein Family: Transcription elongation factor B polypeptide
• UniProt Gene Name: Tceb2
• UniProt Synonym Gene Names: EloB
• UniProt Entry Name: ELOB_RAT

NCBI Description

positive regulatory subunit of the general transcription factor SIII heterotrimer; acts to increast the rate of RNA polymerase II transcription; regulates RNA polymerase II elongation complex activity [RGD, Feb 2006]

Uniprot Description

TCEB2: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Protein type: Transcription initiation complex; Ubiquitin conjugating system; Enzyme, misc.

Cellular Component: Cul2-RING ubiquitin ligase complex; transcription factor complex; Cul5-RING ubiquitin ligase complex; VCB complex; nucleus

Molecular Function: ubiquitin protein ligase binding; ubiquitin-protein ligase activity; protein complex binding

Biological Process: transcription from RNA polymerase II promoter; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter