SERPINA1 sirna

SERPINA1 siRNA (Rat)

Cat. Num. AAA8204764

• Gene Names: Serpina1; Pi; AAT; Spi1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SERPINA1; SERPINA1 siRNA (Rat); Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha-1-proteinase inhibitor; Serpin A1; SERPINA1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SERPINA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 411

• Applicable Applications for SERPINA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SERPINA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SERPINA1 sirna

siRNA to inhibit SERPINA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51036655
• NCBI GeneID: 24648
• NCBI Accession #: NP_071964.2
• NCBI GenBank Nucleotide #: NM_022519.2
• UniProt Accession #: P17475; Q6AYZ5
• Molecular Weight: 46,136 Da
• NCBI Official Full Name: alpha-1-antiproteinase
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• NCBI Official Symbol: Serpina1
• NCBI Official Synonym Symbols: Pi; AAT; Spi1
• NCBI Protein Information: alpha-1-antiproteinase
• UniProt Protein Name: Alpha-1-antiproteinase
• Protein Family: Alpha-1-antitrypsin
• UniProt Gene Name: Serpina1
• UniProt Entry Name: A1AT_RAT

Uniprot Description

SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum

Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; protease binding; endopeptidase inhibitor activity; glycoprotein binding

Biological Process: response to chromate; response to inorganic substance; response to triglyceride; response to cytokine stimulus; response to lead ion; acute-phase response; response to methanol; response to hypoxia; response to lipopolysaccharide; inflammatory response; response to organic cyclic substance; response to estradiol stimulus