anti-Mouse, Rat SERPINA1 Polyclonal Antibody | anti-SERPINA1 antibody

Anti-SERPINA1 Antibody

Cat. Num. AAA178215

• Gene Names: Serpina1; Pi; AAT; Spi1
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: SERPINA1; Polyclonal Antibody; Anti-SERPINA1 Antibody; Alpha-1-antiproteinase; A1A; A1AT; A1AT_HUMAN; AAT; Alpha 1 antiproteinase; Alpha 1 antitrypsin; Alpha 1 antitrypsin null; Alpha 1 protease inhibitor; Alpha-1 protease inhibitor; alpha-1-AT; alpha1 PI; alpha1 proteinase inhibitor; Alpha1AT; Clade A (alpha 1 antiproteinase antitrypsin) member 1; Dom1; MGC23330; MGC9222; PI; PI1; PRO2275; Protease inhibitor 1 (anti elastase); Serine (or cysteine) proteinase inhibitor clade A member 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1; Serpin A1a; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 1; Serpin peptidase inhibitor clade A member 1; SerpinA1; Short peptide from AAT; SPAAT; Spi1-1; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 1; anti-SERPINA1 antibody

• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 411

• Applicable Applications for anti-SERPINA1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived rat SERPINA1 recombinant protein (Position: K211-R411). Rat SERPINA1 shares 66% amino acid (aa) sequence identity with human SERPINA1.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- SERPINA1 Picoband antibody, MBS178215, Western blottingAll lanes: Anti SERPINA1 (MBS178215) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 47KDObserved bind size: 47KD)

Immunohistochemistry (IHC)

(Anti- SERPINA1 Picoband antibody, MBS178215, IHC(P)IHC(P): Rat Spleen Tissue )

Immunohistochemistry (IHC)

(Anti- SERPINA1 Picoband antibody, MBS178215, IHC(P)IHC(P): Mouse Spleen Tissue )

Related Product Information for anti-SERPINA1 antibody

Description: Rabbit IgG polyclonal antibody for Alpha-1-antiproteinase(SERPINA1) detection. Tested with WB, IHC-P in Mouse;Rat.

Background: SERPINA1 is also known as PI, A1A or AAT. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

References

1. Mixture-based combinatorial libraries from small individual peptide libraries: a case study on 1-antitrypsin deficiency.Chang YP, et al. Molecules, 2014 May 16. 2. Serpin peptidase inhibitor clade A member 1 is a biomarker of poor prognosis in gastric cancer.Kwon CH, et al. Br J Cancer, 2014 Nov 11. 3. Alpha 1-antitrypsin therapy mitigated ischemic stroke damage in rats.Moldthan HL, et al. J Stroke Cerebrovasc Dis, 2014 May-Jun.

NCBI and Uniprot Product Information

• NCBI GI #: 51036655
• NCBI GeneID: 24648
• NCBI Accession #: NP_071964.2
• NCBI GenBank Nucleotide #: NM_022519.2
• UniProt Accession #: P17475; Q6AYZ5
• Molecular Weight: 46,136 Da
• NCBI Official Full Name: alpha-1-antiproteinase
• NCBI Official Synonym Full Names: serpin family A member 1
• NCBI Official Symbol: Serpina1
• NCBI Official Synonym Symbols: Pi; AAT; Spi1
• NCBI Protein Information: alpha-1-antiproteinase
• UniProt Protein Name: Alpha-1-antiproteinase
• Protein Family: Alpha-1-antitrypsin
• UniProt Gene Name: Serpina1
• UniProt Entry Name: A1AT_RAT

Uniprot Description

SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus

Molecular Function: endopeptidase inhibitor activity; glycoprotein binding; identical protein binding; protease binding; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; inflammatory response; response to chromate; response to cytokine stimulus; response to estradiol stimulus; response to hypoxia; response to inorganic substance; response to lead ion; response to lipopolysaccharide; response to methanol; response to organic cyclic substance; response to peptide hormone stimulus; response to triglyceride