PCMT1 sirna

PCMT1 siRNA (Rat)

Cat. Num. AAA8202609

• Gene Names: Pcmt1; PCM
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PCMT1; PCMT1 siRNA (Rat); Protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PCMT1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PCMT1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 227

• Applicable Applications for PCMT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PCMT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PCMT1 sirna

siRNA to inhibit PCMT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 56961640
• NCBI GeneID: 25604
• NCBI Accession #: NP_037205.2
• NCBI GenBank Nucleotide #: NM_013073.3
• UniProt Accession #: P22062; Q5M7V6
• Molecular Weight: 24,641 Da
• NCBI Official Full Name: protein-L-isoaspartate(D-aspartate) O-methyltransferase
• NCBI Official Synonym Full Names: protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
• NCBI Official Symbol: Pcmt1
• NCBI Official Synonym Symbols: PCM
• NCBI Protein Information: protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Protein Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Gene Name: Pcmt1
• UniProt Synonym Gene Names: PIMT
• UniProt Entry Name: PIMT_RAT

NCBI Description

catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues [RGD, Feb 2006]

Uniprot Description

PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Transferase; EC 2.1.1.77

Cellular Component: cytoplasm; vesicle

Molecular Function: S-adenosylmethionine-dependent methyltransferase activity; protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

Biological Process: protein amino acid methylation; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process