Rabbit PCMT1 Polyclonal Antibody | anti-PCMT1 antibody

PCMT1 Polyclonal Antibody

Cat. Num. AAA3011669

• Gene Names: PCMT1; PIMT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PCMT1; Polyclonal Antibody; PCMT1 Polyclonal Antibody; Protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; L-isoaspartyl protein carboxyl; methyltransferase; Protein L-isoaspartyl/D-aspartyl; Protein-beta-aspartate methyltransferase; anti-PCMT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PCMT1 polyclonal antibody detects endogenous levels of PCMT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 286

• Applicable Applications for anti-PCMT1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:100; IP: 1:50-1:200
• Immunogen: Recombinant full length Human PCMT1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PCMT1 polyclonal antibody)

Related Product Information for anti-PCMT1 antibody

PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase), also known as PIMT, is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family and is highly expressed in brain. Functioning as a monomer, PCMT localizes to the cytoplasm and participates in the degradation and/or repair of damaged proteins. More specifically, PCMT1 recognizes isomerized Asp or Asn residues in peptides and proteins and catalyzes the conversion of abnormal L-isoaspartyl and D-aspartyl residues to methyl esters that may then spontaneously hydrolyze to re-form normal aspartyl residues. In congruence with this reaction, PCMT1 converts the methyl donor S-adenosylmethionine (AdoMet) to S-andenosylhomocysteine (AdoHcy). In mice lacking PCMT1, damaged proteins accumulate in a variety of tissues and the ratio between AdoMet and AdoHcy is increased in brain tissue. The phenotypic result is progressive epilepsy and death at an early age.

NCBI and Uniprot Product Information

• NCBI GI #: 354983493
• NCBI GeneID: 5110
• NCBI Accession #: NP_001238978.1
• NCBI GenBank Nucleotide #: NP_001238978.1
• UniProt Accession #: P22061
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform 2
• NCBI Official Synonym Full Names: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• NCBI Official Symbol: PCMT1
• NCBI Official Synonym Symbols: PIMT
• NCBI Protein Information: protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Protein Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Gene Name: PCMT1
• UniProt Synonym Gene Names: PIMT
• UniProt Entry Name: PIMT_HUMAN

NCBI Description

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.77; Methyltransferase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: endoplasmic reticulum; cytoplasm; vesicle

Molecular Function: protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

Biological Process: protein repair; protein amino acid methylation