SMYD2 recombinant protein

SMYD2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120681

• Gene Names: SMYD2; KMT3C; HSKM-B; ZMYND14
• Purity: >97% as determined by SDS-PAGE
• Synonyms: SMYD2; SMYD2 Protein; Human; Recombinant (His Tag); Human SMYD2/KMT3C Protein (His Tag); HSKM-B Protein; KMT3C Protein; ZMYND14 Protein; SET and MYND domain containing 2; SMYD2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% glycedrol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His433

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the full length of human SMYD2 (NP_064582.2)(Met 1-His 433) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SMYD2 recombinant protein

Background: SET and MYND domain-containing protein 2, also known as HSKM-B, SMYD2, and KMT3C, is a member of the SMYD protein family. It contains one MYND-type zinc finger and one SET domain. Not much is known about SMYD2. However, the interest in better understanding the roles of SMYD2 has grown because of reports indicating that SMYD2 methylates p53 and histone H3. In Xenopus, SMYD1 and SMYD2 were expressed in various muscle tissues and related to muscle cell differentiation. SMYD2 mRNA is most highly expressed in heart and brain tissue. Over-expressed SMYD2 localizes to the cytoplasm and the nucleus in 293T cells. SMYD2 appears to restrain cell proliferation, likely through direct modulation of chromatin structure. Patients with SMYD2-overexpressing tumors had a worse overall rate of survival than those with non-expressing tumors, and SMYD2 positivity was independently associated with a worse outcome in the multivariate analysis. SMYD2 plays an important role in tumor cell proliferation through its activation/overexpression and regards as a prognosticator and potential therapeutic target in esophageal squamous cell carcinoma (ESCC).

References

Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.

NCBI and Uniprot Product Information

• NCBI GI #: 188035871
• NCBI GeneID: 56950
• NCBI Accession #: NP_064582.2
• NCBI GenBank Nucleotide #: NM_020197
• UniProt Accession #: Q9NRG4; Q4V765; Q5VSH9; Q96AI4; B2R9P9; I6L9H7
• Molecular Weight: 50
• NCBI Official Full Name: N-lysine methyltransferase SMYD2
• NCBI Official Synonym Full Names: SET and MYND domain containing 2
• NCBI Official Symbol: SMYD2
• NCBI Official Synonym Symbols: KMT3C; HSKM-B; ZMYND14
• NCBI Protein Information: N-lysine methyltransferase SMYD2
• UniProt Protein Name: N-lysine methyltransferase SMYD2
• Protein Family: N-lysine methyltransferase
• UniProt Gene Name: SMYD2
• UniProt Synonym Gene Names: KMT3C
• UniProt Entry Name: SMYD2_HUMAN

NCBI Description

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]

Uniprot Description

SMYD2: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Expression is repressed by CEBPA.

Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; p53 binding; metal ion binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA damage response, signal transduction by p53 class mediator