KAT8 sirna

KAT8 siRNA (Rat)

Cat. Num. AAA8227185

• Gene Names: Kat8; Myst1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KAT8; KAT8 siRNA (Rat); MYST1; Histone acetyltransferase KAT8; Lysine acetyltransferase 8; MOZ. YBF2/SAS3. SAS2 and TIP60 protein 1; MYST-1; KAT8 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KAT8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 458

• Applicable Applications for KAT8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KAT8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KAT8 sirna

siRNA to inhibit KAT8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62821766
• NCBI GeneID: 310194
• NCBI Accession #: NP_001017378.1
• NCBI GenBank Nucleotide #: NM_001017378.1
• UniProt Accession #: Q5XI06
• Molecular Weight: 52,632 Da
• NCBI Official Full Name: histone acetyltransferase KAT8
• NCBI Official Synonym Full Names: K (lysine) acetyltransferase 8
• NCBI Official Symbol: Kat8
• NCBI Official Synonym Symbols: Myst1
• NCBI Protein Information: histone acetyltransferase KAT8
• UniProt Protein Name: Histone acetyltransferase KAT8
• Protein Family: KAT8 regulatory NSL complex
• UniProt Gene Name: Kat8
• UniProt Synonym Gene Names: Myst1; MYST-1
• UniProt Entry Name: KAT8_RAT

Uniprot Description

MYST1: Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Belongs to the MYST (SAS/MOZ) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Cellular Component: kinetochore; nuclear membrane; nucleus; histone acetyltransferase complex

Molecular Function: enzyme binding; histone acetyltransferase activity; metal ion binding; acetyltransferase activity; histone acetyltransferase activity (H4-K16 specific); transcription factor binding; methylated histone residue binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of autophagy; myeloid cell differentiation; histone acetylation; negative regulation of transcription, DNA-dependent