JAG1 sirna

JAG1 siRNA (Rat)

Cat. Num. AAA8231551

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: JAG1; JAG1 siRNA (Rat); Protein jagged-1; Jagged1; CD antigen CD339; JAG1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: JAG1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1219

• Applicable Applications for JAG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat JAG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for JAG1 sirna

siRNA to inhibit JAG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9506825
• NCBI GeneID: 29146
• NCBI Accession #: NP_062020.1
• NCBI GenBank Nucleotide #: NM_019147.1
• UniProt Accession #: Q63722; P70640
• Molecular Weight: 134,326 Da
• NCBI Official Full Name: protein jagged-1
• NCBI Official Synonym Full Names: jagged 1
• NCBI Official Symbol: Jag1
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Protein jagged-1
• Protein Family: Protein jagged
• UniProt Gene Name: Jag1
• UniProt Synonym Gene Names: Jagged1
• UniProt Entry Name: JAG1_RAT

NCBI Description

ligand responsible for activating Notch1 [RGD, Feb 2006]

Uniprot Description

JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Protein type: Membrane protein, integral

Cellular Component: adherens junction; apical part of cell; apical plasma membrane; plasma membrane

Molecular Function: Notch binding; protein binding

Biological Process: auditory receptor cell differentiation; blood vessel remodeling; camera-type eye development; inner ear development; morphogenesis of an epithelial sheet; negative regulation of cell differentiation; negative regulation of fat cell differentiation; negative regulation of neuron differentiation; Notch signaling pathway; organ morphogenesis; organ regeneration; positive regulation of myeloid cell differentiation; positive regulation of Notch signaling pathway; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; response to muramyl dipeptide; T cell mediated immunity