Rat Stem Cell Factor (SCF) RTU ELISA Kit | SCF rtu elisa kit

Rat Stem Cell Factor (SCF) ELISA Kit

Cat. Num. AAA4502373

• Gene Names: Kitlg; Mgf; SCF; Kitl
• Reactivity: Rat
• Synonyms: Stem Cell Factor (SCF); Rat Stem Cell Factor (SCF) ELISA Kit; KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2; SCF rtu elisa kit

• Reactivity: Rat
• Sequence Length: 273

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.4pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCF rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SCF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 33667068
• NCBI GeneID: 60427
• NCBI Accession #: NP_068615.1
• NCBI GenBank Nucleotide #: NM_021843.4
• UniProt Accession #: P21581; Q9QWZ4; Q9Z2E7
• Molecular Weight: 27,681 Da
• NCBI Official Full Name: kit ligand isoform 1
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: Kitlg
• NCBI Official Synonym Symbols: Mgf; SCF; Kitl
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• UniProt Gene Name: Kitlg
• UniProt Synonym Gene Names: Kitl; Mgf; MGF; SCF; sKITLG

NCBI Description

soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development [RGD, Feb 2006]

Uniprot Description

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.