ENPP1 sirna

ENPP1 siRNA (Rat)

Cat. Num. AAA8206610

• Gene Names: Enpp1; Pc1; Npps
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENPP1; ENPP1 siRNA (Rat); NPPS; PC1; PDNP1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1; ENPP1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ENPP1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 905

• Applicable Applications for ENPP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ENPP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENPP1 sirna

siRNA to inhibit ENPP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758300
• NCBI GeneID: 85496
• NCBI Accession #: NP_445987.1
• NCBI GenBank Nucleotide #: NM_053535.1
• UniProt Accession #: Q924C3; Q91XQ3; Q920C8
• Molecular Weight: 102,843 Da
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 1
• NCBI Official Symbol: Enpp1
• NCBI Official Synonym Symbols: Pc1; Npps
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: Enpp1
• UniProt Synonym Gene Names: Npps; Pc1; Pdnp1; E-NPP 1; NPPase
• UniProt Entry Name: ENPP1_RAT

NCBI Description

mutation of the human homolog is detected in patients with idiopathic infantile arterial calcification [RGD, Feb 2006]

Uniprot Description

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Phosphatase (non-protein); Carbohydrate Metabolism - starch and sucrose; Phosphodiesterase; EC 3.6.1.9; EC 3.1.4.1; Cofactor and Vitamin Metabolism - riboflavin

Cellular Component: extracellular space; cell surface; integral to plasma membrane; basolateral plasma membrane; lysosomal membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: phosphodiesterase I activity; nucleotide diphosphatase activity; protein homodimerization activity; nucleic acid binding; zinc ion binding; metal ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; scavenger receptor activity; ATP binding; insulin receptor binding; polysaccharide binding

Biological Process: receptor-mediated endocytosis; sequestering of triacylglycerol; generation of precursor metabolites and energy; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; negative regulation of fat cell differentiation; bone remodeling; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process