Standard Curve (Sample)
Human Melatonin receptor type 1A (MTNR1A) ELISA Kit | MTNR1A elisa kit
Human Melatonin receptor type 1A (MTNR1A) ELISA Kit
Gene Names
MTNR1A; MT1; MEL-1A-R
Reactivity
Human
Synonyms
Melatonin receptor type 1A (MTNR1A); N/A; Human Melatonin receptor type 1A (MTNR1A) ELISA Kit; MTNR1A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of MTNR1A. No significant cross-reactivity or interference between MTNR1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MTNR1A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MTNR1A elisa kit
Intended Uses: This MTNR1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanMTNR1A. This ELISA kit for research use only!
Principle of the Assay: MTNR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MTNR1A antibody and an MTNR1A-HRP conjugate. The assay sample and buffer are incubated together with MTNR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTNR1A concentration since MTNR1A from samples and MTNR1A-HRP conjugate compete for the anti-MTNR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by MTNR1A from the sample, fewer sites are left to bind MTNR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTNR1A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MTNR1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MTNR1A antibody and an MTNR1A-HRP conjugate. The assay sample and buffer are incubated together with MTNR1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTNR1A concentration since MTNR1A from samples and MTNR1A-HRP conjugate compete for the anti-MTNR1A antibody binding site. Since the number of sites is limited, as more sites are occupied by MTNR1A from the sample, fewer sites are left to bind MTNR1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTNR1A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MTNR1A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,375 Da
NCBI Official Full Name
melatonin receptor type 1A
NCBI Official Synonym Full Names
melatonin receptor 1A
NCBI Official Symbol
MTNR1A
NCBI Official Synonym Symbols
MT1; MEL-1A-R
NCBI Protein Information
melatonin receptor type 1A; mel1a receptor
UniProt Protein Name
Melatonin receptor type 1A
UniProt Gene Name
MTNR1A
UniProt Synonym Gene Names
Mel-1A-R; Mel1a receptor
UniProt Entry Name
MTR1A_HUMAN
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Product Notes
The Human MTNR1A mtnr1a (Catalog #AAA245805) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245805 ELISA Kit recognizes Human MTNR1A. It is sometimes possible for the material contained within the vial of "Melatonin receptor type 1A (MTNR1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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