Immunohistochemistry (IHC)
(Anti-CD56 antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Mouse anti-Human NCAM / CD56 Monoclonal Antibody | anti-NCAM antibody
Mouse Monoclonal [clone 123C3] (IgG1) to Human NCAM / CD56
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified
Synonyms
NCAM / CD56; Monoclonal Antibody; Mouse Monoclonal [clone 123C3] (IgG1) to Human NCAM / CD56; Anti-NCAM / CD56 Antibody (clone 123C3) IHC-plus; NCAM1; CD56; CD56 antigen; MSK39; NCAM-1; N-CAM-1; NCAM; Human NCAM; anti-NCAM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
123C3
Specificity
The antibody recognizes a transmembrane glycoprotein of 140 and 180 kD which has been identified as NCAM (Neural Cell Adhesion Module). It is involved in intercellular adhesion and play a role in outgrowth of neurites and the development of the nervous system. Several other isoforms have been identified which are expressed in a developmental and tissue specific pattern. Two major epitopes have been defined, NKI-nbl-1 (MON 9012) reacts with epitope 1 and 123C3 (MON 9006) reacts with epitope 2. At the international Workshop on SCLC antibodies 123C3 has been categorized as cluster 1 antibody (Stahel et al 1994). Most importantly, 123C3 can be used on routing formalin fixed paraffin section after retrieval. All cells in small cell carcinomas and carcinoids of the lung are strongly positive for 123C3. A minority of cases of other major types of lung carcinoma are sometimes positive as well: however this positivity is generally weak and focal. Adenoid cystic carcinomas of bronchial glands are strongly positive. Neuroblastoma's and Wilms tumors are usually also staining strongly positive. In non small lung cell carcinomas, 123C3 staining has been associated with more advanced stage and a decreased survival after surgery (Kibbelaar et al. 1991). Positive staining with other tumors, include medullary thyroid carcinomas and some ovarian tumors. NCAM (CD56) is a marker for natural killer: 123C3 can be used to distinguish NK cells from other hematopoietic cell populations.
Purity/Purification
Purified
Form/Format
PBS, 0.02% sodium azide, 0.1% BSA
Concentration
0.1 mg/ml (varies by lot)
Sequence Length
848
Applicable Applications for anti-NCAM antibody
Immunohistochemistry (IHC - Paraffin, IHC - Frozen), Immunofluorescence (IF)
Application Notes
IF, IHC-Fr, IHC-P (1:100)
Usage: Study of small cell carcinoma. Detection of CD56 positive lymphomas. For immunofluorescence and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dil...
Usage: Study of small cell carcinoma. Detection of CD56 positive lymphomas. For immunofluorescence and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dil...
Target Species
Human
Preparation and Storage
Store at 4 degree C.
Immunohistochemistry (IHC)
(Anti-CD56 antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC)
(Anti-CD56 antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC)
(Anti-CD56 antibody IHC of human intestine, ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC)
(Anti-CD56 antibody IHC of human intestine, ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Product Categories/Family for anti-NCAM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,760 Da
NCBI Official Full Name
neural cell adhesion molecule 1 isoform 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
NCAM1
NCBI Official Synonym Symbols
CD56; NCAM; MSK39
NCBI Protein Information
neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal antibody 5.1H11
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Gene Name
NCAM1
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_HUMAN
Similar Products
Product Notes
The NCAM ncam1 (Catalog #AAA245805) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 123C3] (IgG1) to Human NCAM / CD56 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCAM / CD56 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin, IHC - Frozen), Immunofluorescence (IF). IF, IHC-Fr, IHC-P (1:100) Usage: Study of small cell carcinoma. Detection of CD56 positive lymphomas. For immunofluorescence and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dil.. Researchers should empirically determine the suitability of the NCAM ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCAM / CD56, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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