Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA Kit | Enpp1 elisa kit

Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA Kit

Cat. Num. AAA762772

• Gene Names: ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• Reactivity: Rat
• Synonyms: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Rat Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ELISA Kit; Enpp1/Phosphodiesterase I/nucleotide pyrophosphatase 1/Plasma-cell membrane glycoprotein PC-1/; Enpp1 elisa kit

• Reactivity: Rat
• Sequence Length: 925

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Enpp1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Enpp1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Enpp1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Enpp1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Enpp1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 170650661
• NCBI GeneID: 5167
• NCBI Accession #: NP_006199.2
• NCBI GenBank Nucleotide #: NM_006208.2
• Molecular Weight: 104,924 Da
• NCBI Official Full Name: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 1
• NCBI Official Symbol: ENPP1
• NCBI Official Synonym Symbols: M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
• Protein Family: Ectonucleotide pyrophosphatase/phosphodiesterase family
• UniProt Gene Name: ENPP1
• UniProt Synonym Gene Names: M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase
• UniProt Entry Name: ENPP1_HUMAN

NCBI Description

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Nucleotide Metabolism - purine; Carbohydrate Metabolism - starch and sucrose; EC 3.6.1.9; Phosphatase (non-protein); Motility/polarity/chemotaxis; EC 3.1.4.1; Cofactor and Vitamin Metabolism - riboflavin; Membrane protein, integral; Phosphodiesterase; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: basolateral plasma membrane; cell surface; extracellular space; integral to membrane; integral to plasma membrane; lysosomal membrane; plasma membrane

Molecular Function: 3'-phosphoadenosine 5'-phosphosulfate binding; ATP binding; calcium ion binding; insulin receptor binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein binding; protein homodimerization activity; scavenger receptor activity; zinc ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; biomineral formation; cellular phosphate ion homeostasis; cellular response to insulin stimulus; generation of precursor metabolites and energy; immune response; inorganic diphosphate transport; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; nucleoside triphosphate catabolic process; phosphate metabolic process; receptor-mediated endocytosis; regulation of bone mineralization; riboflavin metabolic process; sequestering of triacylglycerol; vitamin metabolic process; water-soluble vitamin metabolic process

Disease: Arterial Calcification, Generalized, Of Infancy, 1; Cole Disease; Diabetes Mellitus, Noninsulin-dependent; Hypophosphatemic Rickets, Autosomal Recessive, 2; Obesity