Microtubule Associated Protein Tau Recombinant Protein | MAPt recombinant protein

Recombinant Microtubule Associated Protein Tau (MAPt)

Cat. Num. AAA2030402

• Gene Names: MAPT; tau
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Microtubule Associated Protein Tau; Recombinant Microtubule Associated Protein Tau (MAPt); MAPt recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K VVQEGFLREPGPPGLSHQLM SGMPGAPLLP EGPREATRQP SGTGPEDTEG GRHAPELLKH QLLGDLHQEG PPLKGAGGKE RPGSKEEVDE DRDVDESSPQ DSPPSKASPA QDGRPPQTAA REATSIPGFP AEGAIPLPVD FLSKVSTEIP ASEPDGPSVG RAKGQDAPLE FTFHVEITPN VQKEQAHSEE HLGRAAFPGA PGEGPEARGP SLGEDTKEAD LPEPSEKQPAAAPRGKPVSR VPQLKARMVSKSKDGT
• Sequence Length: 448

• Applicable Applications for MAPt recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.7kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Lys130~Thr386
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 27806547
• NCBI GeneID: 281296
• NCBI Accession #: NP_776531.1
• NCBI GenBank Nucleotide #: NM_174106.2
• Molecular Weight: 43,806 Da
• NCBI Official Full Name: microtubule-associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: tau
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: TAU; PHF-tau
• UniProt Entry Name: TAU_BOVIN

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Cellular Component: microtubule; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol

Molecular Function: protein binding; microtubule binding; apolipoprotein binding; protein kinase binding; SH3 domain binding

Biological Process: negative regulation of intracellular transport; axon extension; positive regulation of axon extension; positive regulation of microtubule polymerization; neuron migration; axon cargo transport; regulation of autophagy; microtubule cytoskeleton organization and biogenesis; adult walking behavior; mitochondrion transport along microtubule