DLG3 sirna

DLG3 siRNA (Rat)

Cat. Num. AAA8226925

• Gene Names: Dlg3; Dlgh3
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLG3; DLG3 siRNA (Rat); DLGH3; Disks large homolog 3; PSD-95/SAP90-related protein 1; Synapse-associated protein 102; SAP-102; SAP102; DLG3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DLG3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 835

• Applicable Applications for DLG3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DLG3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLG3 sirna

siRNA to inhibit DLG3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13928878
• NCBI GeneID: 58948
• NCBI Accession #: NP_113827.1
• NCBI GenBank Nucleotide #: NM_031639.1
• UniProt Accession #: Q62936; P70547
• Molecular Weight: 92,172 Da
• NCBI Official Full Name: disks large homolog 3
• NCBI Official Synonym Full Names: discs, large homolog 3 (Drosophila)
• NCBI Official Symbol: Dlg3
• NCBI Official Synonym Symbols: Dlgh3
• NCBI Protein Information: disks large homolog 3
• UniProt Protein Name: Disks large homolog 3
• Protein Family: Disks large
• UniProt Gene Name: Dlg3
• UniProt Synonym Gene Names: Dlgh3; SAP-102; SAP102
• UniProt Entry Name: DLG3_RAT

NCBI Description

human homolog is a protein with guanylate kinase activity; involved in synaptogenesis and cell proliferation [RGD, Feb 2006]

Uniprot Description

DLG3: Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling. Interacts through its PDZ domains with NETO1, GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4. Interacts through its PDZ domains with APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: postsynaptic membrane; extracellular space; tight junction; growth cone; cell soma; basolateral plasma membrane; cytoplasm; postsynaptic density; plasma membrane; ionotropic glutamate receptor complex; synapse; intercellular junction; dendritic shaft

Molecular Function: protein C-terminus binding; guanylate kinase activity; protein domain specific binding; ionotropic glutamate receptor binding; protein binding; ubiquitin protein ligase binding; protein phosphatase binding; PDZ domain binding; phosphatase binding

Biological Process: establishment and/or maintenance of epithelial cell polarity; nervous system development; synaptic transmission; establishment of planar polarity; receptor clustering; nucleotide phosphorylation