Western Blot (WB)
(Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Mouse SAP102 Monoclonal Antibody | anti-Dlg3 antibody
SAP102 Antibody
Western Blot (WB)
(Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western Blot analysis of Rat brain lysates showing detection of SAP102 protein using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Scientific Background: Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homology 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
NCBI and Uniprot Product Information
NCBI Description
human homolog is a protein with guanylate kinase activity; involved in synaptogenesis and cell proliferation [RGD, Feb 2006]
Uniprot Description
DLG3: Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling. Interacts through its PDZ domains with NETO1, GRIN2B and SYNGAP1. Interacts through its guanylate kinase-like domain with DLGAP1, DLGAP2, DLGAP3 and DLGAP4. Interacts through its PDZ domains with APC. Interacts through its first two PDZ domains with ERBB4. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Cellular Component: postsynaptic membrane; extracellular space; growth cone; tight junction; cell soma; basolateral plasma membrane; cytoplasm; postsynaptic density; plasma membrane; ionotropic glutamate receptor complex; synapse; intercellular junction; dendritic shaft
Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein domain specific binding; protein binding; ubiquitin protein ligase binding; protein phosphatase binding; phosphatase binding; PDZ domain binding
Biological Process: establishment and/or maintenance of epithelial cell polarity; nervous system development; synaptic transmission; establishment of planar polarity; receptor clustering; nucleotide phosphorylation
Research Articles on Dlg3
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Product Notes
The Dlg3 dlg3 (Catalog #AAA801834) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SAP102 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SAP102 can be used in a range of immunoassay formats including, but not limited to, Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1000 (ECL). Researchers should empirically determine the suitability of the Dlg3 dlg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP102, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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