CEBPD sirna

CEBPD siRNA (Rat)

Cat. Num. AAA8202195

• Gene Names: Cebpd; CELF; C/EBPd
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CEBPD; CEBPD siRNA (Rat); CELF; CCAAT/enhancer-binding protein delta; C/EBP delta; Transcription factor CELF; CEBPD sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CEBPD siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 268

• Applicable Applications for CEBPD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CEBPD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4°C. Store at -20°C for one year.

Related Product Information for CEBPD sirna

siRNA to inhibit CEBPD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978643
• NCBI GeneID: 25695
• NCBI Accession #: NP_037286.1
• NCBI GenBank Nucleotide #: NM_013154.2
• UniProt Accession #: Q03484
• NCBI Official Full Name: CCAAT/enhancer-binding protein delta
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), delta
• NCBI Official Symbol: Cebpd
• NCBI Official Synonym Symbols: CELF; C/EBPd
• NCBI Protein Information: CCAAT/enhancer-binding protein delta
• UniProt Protein Name: CCAAT/enhancer-binding protein delta
• Protein Family: CCAAT/enhancer-binding protein
• UniProt Gene Name: Cebpd
• UniProt Synonym Gene Names: Celf; C/EBP delta
• UniProt Entry Name: CEBPD_RAT

NCBI Description

transcriptional activator; activity is stimulated by sepsis, keratinocyte growth factor, and may be involved in glucose-mediated regulation of iNOS [RGD, Feb 2006]

Uniprot Description

C/EBP-delta: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha. Interacts with SPI1/PU.1. Interacts with PRDM16. Belongs to the bZIP family. C/EBP subfamily.

Protein type: DNA-binding; Motility/polarity/chemotaxis

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity

Biological Process: fat cell differentiation; regulation of transcription from RNA polymerase II promoter; positive regulation of osteoblast differentiation; transcription, DNA-dependent; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inner ear development