ADH1C recombinant protein

ADH1C, 1-375aa, Human, His tag, E Coli

Cat. Num. AAA204332

• Gene Names: ADH1C; ADH3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ADH1C; 1-375aa; Human; His tag; E Coli; alcohol dehydrogenase 1C; ADH3; ADH1C recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 10% glycerol, 2mM DTT, 200mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSTAGK VIKCKAAVLW ELKKPFSIEE VEVAPPKAHE VRIKMVAAGI CRSDEHVVSG NLVTPLPVIL GHEAAGIVES VGEGVTTVKP GDKVIPLFTP QCGKCRICKN PESNYCLKND LGNPRGTLQD GTRRFTCSGK PIHHFVGVST FSQYTVVDEN AVAKIDAASP LEKVCLIGCG FSTGYGSAVK VAKVTPGSTC AVFGLGGVGL SVVMGCKAAG AARIIAVDIN KDKFAKAKEL GATECINPQD YKKPIQEVLK EMTDGGVDFS FEVIGRLDTM MASLLCCHEA CGTSVIVGVP PDSQNLSINP MLLLTGRTWK GAIFGGFKSK ESVPKLVADF MAKKFSLDAL ITNILPFEKI NEGFDLLRSG KSIRTVLTF
• Sequence Length: 375

• Applicable Applications for ADH1C recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ADH1C recombinant protein

Alcohol dehydrogenase 1C, also known as ADH1C, belongs to the zinc-containing alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. ADH1 is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. Recombinant human ADH1C protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ADH1C recombinant protein

Redox Proteins

References

Jelski W., et al. (2007) Dig Dis Sci. 52:1513-1516.; Smith M., et al. (1973) Ann Hum Genet. 36:401-414.

NCBI and Uniprot Product Information

• NCBI GI #: 4501933
• NCBI GeneID: 126
• NCBI Accession #: NP_000660
• NCBI GenBank Nucleotide #: NM_000669.4
• UniProt Accession #: P00326; Q4PJ18; Q5WRV0; Q6LBW4; Q6NWV0; Q6NZA7
• Molecular Weight: 42.4kDa (399aa) confirmed by MALDI-TOF
• NCBI Official Full Name: alcohol dehydrogenase 1C
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1C (class I), gamma polypeptide
• NCBI Official Symbol: ADH1C
• NCBI Official Synonym Symbols: ADH3
• NCBI Protein Information: alcohol dehydrogenase 1C
• UniProt Protein Name: Alcohol dehydrogenase 1C
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH1C
• UniProt Synonym Gene Names: ADH3

NCBI Description

This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

MiscellaneousThere are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.