Propionyl Coenzyme A Carboxylase Beta Recombinant Protein | PCCb recombinant protein

Recombinant Propionyl Coenzyme A Carboxylase Beta (PCCb)

Cat. Num. AAA2034558

• Reactivity: Mouse
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Propionyl Coenzyme A Carboxylase Beta; Recombinant Propionyl Coenzyme A Carboxylase Beta (PCCb); Propanoyl-CoA:carbon dioxide ligase subunit beta; Propionyl-CoA carboxylase beta chain; mitochondrial; PCCb recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Mouse
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 559

• Applicable Applications for PCCb recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Glu311~Leu541
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Glu311~Leu541 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119599524
• NCBI GeneID: 5096
• Molecular Weight: Predicted: 29.0kDa
• NCBI Official Full Name: propionyl Coenzyme A carboxylase, beta polypeptide, isoform CRA_c
• NCBI Official Synonym Full Names: propionyl-CoA carboxylase beta subunit
• NCBI Official Symbol: PCCB
• NCBI Protein Information: propionyl-CoA carboxylase beta chain, mitochondrial
• UniProt Protein Name: Propionyl-CoA carboxylase beta chain, mitochondrial
• Protein Family: Phycoerythrocyanin
• UniProt Gene Name: PCCB
• UniProt Synonym Gene Names: PCCase subunit beta
• UniProt Entry Name: PCCB_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

PCCB: Defects in PCCB are the cause of propionic acidemia type II (PA-2). PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. Belongs to the AccD/PCCB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial; Carbohydrate Metabolism - propanoate; EC 6.4.1.3; Ligase

Chromosomal Location of Human Ortholog: 3q21-q22

Cellular Component: cytosol; mitochondrial matrix; mitochondrion

Biological Process: biotin metabolic process; short-chain fatty acid catabolic process

Disease: Propionic Acidemia