CCL20 sirna

CCL20 siRNA (Rat)

Cat. Num. AAA8217160

• Gene Names: Ccl20; ST38; Scya20
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCL20; CCL20 siRNA (Rat); SCYA20; ST38; C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20; CCL20 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CCL20 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 96

• Applicable Applications for CCL20 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CCL20 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCL20 sirna

siRNA to inhibit CCL20 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9507069
• NCBI GeneID: 29538
• NCBI Accession #: NP_062106.1
• NCBI GenBank Nucleotide #: NM_019233.1
• UniProt Accession #: P97884
• Molecular Weight: 10,875 Da
• NCBI Official Full Name: C-C motif chemokine 20
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: Ccl20
• NCBI Official Synonym Symbols: ST38; Scya20
• NCBI Protein Information: C-C motif chemokine 20
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl20
• UniProt Synonym Gene Names: Scya20; St38; MIP-3-alpha
• UniProt Entry Name: CCL20_RAT

NCBI Description

chemokine upregulated in ischemic brain tissue [RGD, Feb 2006]

Uniprot Description

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.