Rat MIP-3 alpha/CCL20 ELISA Kit | CCL20 elisa kit

Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit

Cat. Num. AAA175952

• Gene Names: Ccl20; ST38; Scya20
• Reactivity: Rat
• Synonyms: MIP-3 alpha/CCL20; Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; Beta chemokine exodus 1; Beta-chemokine exodus-1; C C motif chemokine 20; CC chemokine LARC; Ccl20; CCL20(2-70); CCL20_HUMAN; Chemokine (C C motif) ligand 20; CKb4; Exodus 1; LARC; Liver and activation regulated chemokine; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP 3 alpha; MIP 3a; MIP-3-alpha; MIP3 alpha; MIP3A; MIP3alpha; SCYA20; Small inducible cytokine A20; Small inducible cytokine subfamily A (Cys Cys) member 20; Small-inducible cytokine A20; ST38; CCL20 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Ccl20
• Sequence Length: 96

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL20 elisa kit

Principle of the Assay: The Picokine™ Rat Ccl20 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ccl20 with a 96-well strip plate that is pre-coated with antibody specific for Ccl20. The detection antibody is a biotinylated antibody specific for Ccl20. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ccl20 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S27-M96. The kit is analytically validated with ready to use reagents. To measure Rat Ccl20, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Ccl20 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ccl20 in the sample.

Background/Introduction: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

NCBI and Uniprot Product Information

• NCBI GI #: 6094385
• NCBI GeneID: 29538
• UniProt Accession #: P97884
• Molecular Weight: 10,875 Da
• NCBI Official Full Name: C-C motif chemokine 20
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: Ccl20
• NCBI Official Synonym Symbols: ST38; Scya20
• NCBI Protein Information: C-C motif chemokine 20; MIP-3-alpha; CC chemokine LARC; CC chemokine ST38; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; small inducible cytokine subfamily A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl20
• UniProt Synonym Gene Names: Scya20; St38; MIP-3-alpha
• UniProt Entry Name: CCL20_RAT

NCBI Description

chemokine upregulated in ischemic brain tissue [RGD, Feb 2006]

Uniprot Description

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.