BSG sirna

BSG siRNA (Rat)

Cat. Num. AAA8212977

• Gene Names: Bsg; CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BSG; BSG siRNA (Rat); Basigin; Glycoprotein CE9; OX-47 antigen; CD antigen CD147; BSG sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: BSG siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 388

• Applicable Applications for BSG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BSG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice. Store at -20 degree C for one year.

Related Product Information for BSG sirna

siRNA to inhibit BSG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158081773
• NCBI GeneID: 25246
• NCBI Accession #: NP_001103352.1
• NCBI GenBank Nucleotide #: NM_001109882.1
• UniProt Accession #: P26453; Q7TNP1
• NCBI Official Full Name: basigin isoform 1
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: Bsg
• NCBI Official Synonym Symbols: CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin
• NCBI Protein Information: basigin
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: Bsg
• UniProt Entry Name: BASI_RAT

NCBI Description

MRC OX-47 antigen that is upregulated on activated lymphocytes; member of the immunoglobulin superfamily [RGD, Feb 2006]

Uniprot Description

BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: focal adhesion; membrane; mitochondrion; integral to plasma membrane; plasma membrane; melanosome; sarcolemma; lipid raft; acrosomal membrane

Molecular Function: mannose binding; protein binding

Biological Process: response to mercury ion; response to peptide hormone stimulus; response to cAMP; decidualization; embryo implantation; odontogenesis of dentine-containing teeth