BCAT2 sirna

BCAT2 siRNA (Rat)

Cat. Num. AAA8206834

• Gene Names: Bcat2; BCT2; Bcatm; mBcat
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCAT2; BCAT2 siRNA (Rat); BCATM; ECA40; Branched-chain-amino-acid aminotransferase mitochondrial; BCAT(m); BCAT2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: BCAT2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 393

• Applicable Applications for BCAT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BCAT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCAT2 sirna

siRNA to inhibit BCAT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11693174
• NCBI GeneID: 64203
• NCBI Accession #: NP_071795.1
• NCBI GenBank Nucleotide #: NM_022400.1
• UniProt Accession #: O35854
• Molecular Weight: 44,276 Da
• NCBI Official Full Name: branched-chain-amino-acid aminotransferase, mitochondrial
• NCBI Official Synonym Full Names: branched chain amino acid transaminase 2, mitochondrial
• NCBI Official Symbol: Bcat2
• NCBI Official Synonym Symbols: BCT2; Bcatm; mBcat
• NCBI Protein Information: branched-chain-amino-acid aminotransferase, mitochondrial
• UniProt Protein Name: Branched-chain-amino-acid aminotransferase, mitochondrial
• UniProt Gene Name: Bcat2
• UniProt Synonym Gene Names: Bcatm; Eca40; BCAT(m)
• UniProt Entry Name: BCAT2_RAT

NCBI Description

mitochondrial branched-chain-amino-acid transaminase [RGD, Feb 2006]

Uniprot Description

BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.6.1.42; Transferase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: branched-chain-amino-acid transaminase activity

Biological Process: lactation; isoleucine metabolic process; valine metabolic process; leucine biosynthetic process; branched chain family amino acid catabolic process; leucine metabolic process; branched chain family amino acid metabolic process; isoleucine catabolic process; branched chain family amino acid biosynthetic process