Typical Testing Data/Standard Curve
Rat Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) ELISA Kit | BCAT2 elisa kit
Rat Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||BCAT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCAT2 antibody and an BCAT2-HRP conjugate. The assay sample and buffer are incubated together with BCAT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCAT2 concentration since BCAT2 from samples and BCAT2-HRP conjugate compete for the anti-BCAT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCAT2 from the sample, fewer sites are left to bind BCAT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCAT2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
mitochondrial branched-chain-amino-acid transaminase [RGD, Feb 2006]
Uniprot Description
BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.6.1.42; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Transferase; Mitochondrial
Cellular Component: mitochondrion; cytoplasm; nucleus
Molecular Function: branched-chain-amino-acid transaminase activity
Biological Process: lactation; isoleucine metabolic process; valine metabolic process; leucine biosynthetic process; branched chain family amino acid catabolic process; leucine metabolic process; branched chain family amino acid metabolic process; branched chain family amino acid biosynthetic process; isoleucine catabolic process
Research Articles on BCAT2
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Product Notes
The Rat BCAT2 bcat2 (Catalog #AAA7227842) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227842 ELISA Kit recognizes Rat BCAT2. It is sometimes possible for the material contained within the vial of "Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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