Typical Testing Data/Standard Curve (for reference only)
Bovine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/CCL2/MCAF ELISA Kit | MCP-1 elisa kit
Bovine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/CCL2/MCAF ELISA Kit
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Bovine
Synonyms
Monocyte chemotactic protein 1/monocyte chemotactic and activating factor; MCP-1/CCL2/MCAF; Bovine Monocyte chemotactic protein 1/monocyte chemotactic and activating factor; MCP-1/CCL2/MCAF ELISA Kit; MCP-1 elisa kit
Reactivity
Bovine
Sequence Length
99
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
50ng/L - 10000ng/L
Sensitivity
26.13ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MCP-1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Bovine Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor (also known as MCP-1/CCL2/MCAF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine MCP-1/CCL2/MCAF antibody. MCP-1/CCL2/MCAF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine MCP-1/CCL2/MCAF Antibody is added and binds to MCP-1/CCL2/MCAF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MCP-1/CCL2/MCAF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine MCP-1/CCL2/MCAF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine MCP-1/CCL2/MCAF antibody. MCP-1/CCL2/MCAF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine MCP-1/CCL2/MCAF Antibody is added and binds to MCP-1/CCL2/MCAF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MCP-1/CCL2/MCAF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine MCP-1/CCL2/MCAF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
MCP-1
NCBI Official Synonym Full Names
C-C motif chemokine ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2
Protein Family
NCBI Description
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Research Articles on MCP-1
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Product Notes
The Bovine MCP-1 (Catalog #AAA1605305) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1605305 ELISA Kit recognizes Bovine MCP-1. It is sometimes possible for the material contained within the vial of "Monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/CCL2/MCAF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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