ZRANB3 sirna

ZRANB3 siRNA (Mouse)

Cat. Num. AAA8202365

• Gene Names: Zranb3; AH2; AI316834; C730006D09; 4933425L19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZRANB3; ZRANB3 siRNA (Mouse); DNA annealing helicase and endonuclease ZRANB3; Annealing helicase 2; AH2; Zinc finger Ran-binding domain-containing protein 3; ZRANB3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZRANB3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1073

• Applicable Applications for ZRANB3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZRANB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZRANB3 sirna

siRNA to inhibit ZRANB3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 553727082
• NCBI GeneID: 226409
• NCBI Accession #: NP_001272874.1
• NCBI GenBank Nucleotide #: NM_001285945.1
• UniProt Accession #: Q6NZP1; Q148X9; Q8BJJ3; Q8BWT6
• Molecular Weight: 120,896 Da
• NCBI Official Full Name: DNA annealing helicase and endonuclease ZRANB3 isoform 2
• NCBI Official Synonym Full Names: zinc finger, RAN-binding domain containing 3
• NCBI Official Symbol: Zranb3
• NCBI Official Synonym Symbols: AH2; AI316834; C730006D09; 4933425L19Rik
• NCBI Protein Information: DNA annealing helicase and endonuclease ZRANB3
• UniProt Protein Name: DNA annealing helicase and endonuclease ZRANB3
• Protein Family: DNA annealing helicase and endonuclease
• UniProt Gene Name: Zranb3
• UniProt Synonym Gene Names: AH2
• UniProt Entry Name: ZRAB3_MOUSE

Uniprot Description

ZRANB3: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks. Belongs to the SNF2/RAD54 helicase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.-; Helicase; EC 3.6.1.-

Cellular Component: nuclear replication fork; chromosome; nucleus

Molecular Function: nuclease activity; hydrolase activity; zinc ion binding; metal ion binding; nucleotide binding; helicase activity; T/G mismatch-specific endonuclease activity; phosphoric ester hydrolase activity; endodeoxyribonuclease activity; DNA binding; nucleic acid binding; endonuclease activity; catalytic activity; ATP binding

Biological Process: negative regulation of DNA recombination; metabolic process; DNA strand renaturation; replication fork protection; DNA repair; response to DNA damage stimulus; DNA catabolic process, endonucleolytic; replication fork processing; response to UV