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Western Blot (WB) (Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.)

CAF1B Antibody | anti-CAF1B antibody

CAF1B Antibody

Gene Names
CHAF1B; CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CAF1B; CAF1B Antibody; CAF-1 subunit B; Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-Ip60; M-phase phosphoprotein 7; anti-CAF1B antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
CAF1B antibody detects endogenous levels of total CAF1B protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
559
Applicable Applications for anti-CAF1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human CAF1B.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.)

Western Blot (WB) (Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.)
References
Kaufman P.D., Cell 81:1105-1114(1995). Hattori M., Nature 405:311-319(2000). Matsumoto-Taniura N., Mol. Biol. Cell 7:1455-1469(1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,493 Da
NCBI Official Full Name
chromatin assembly factor 1 subunit B
NCBI Official Synonym Full Names
chromatin assembly factor 1 subunit B
NCBI Official Symbol
CHAF1B
NCBI Official Synonym Symbols
CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
NCBI Protein Information
chromatin assembly factor 1 subunit B
UniProt Protein Name
Chromatin assembly factor 1 subunit B
UniProt Gene Name
CHAF1B
UniProt Synonym Gene Names
CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60

NCBI Description

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

Uniprot Description

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Research Articles on CAF1B

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Product Notes

The CAF1B chaf1b (Catalog #AAA854772) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CAF1B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CAF1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the CAF1B chaf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAF1B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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