ZIC1 sirna

ZIC1 siRNA (Mouse)

Cat. Num. AAA8222280

• Gene Names: Zic1; ZIC; ZNF201
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZIC1; ZIC1 siRNA (Mouse); ZIC; Zinc finger protein ZIC 1; Zinc finger protein of the cerebellum 1; ZIC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZIC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 447

• Applicable Applications for ZIC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZIC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZIC1 sirna

siRNA to inhibit ZIC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42476265
• NCBI GeneID: 22771
• NCBI Accession #: NP_033599.2
• NCBI GenBank Nucleotide #: NM_009573.3
• UniProt Accession #: P46684; Q6PAK5; Q80Y18
• Molecular Weight: 48,331 Da
• NCBI Official Full Name: zinc finger protein ZIC 1
• NCBI Official Synonym Full Names: zinc finger protein of the cerebellum 1
• NCBI Official Symbol: Zic1
• NCBI Official Synonym Symbols: ZIC; ZNF201
• NCBI Protein Information: zinc finger protein ZIC 1
• UniProt Protein Name: Zinc finger protein ZIC 1
• Protein Family: Zinc finger protein
• UniProt Gene Name: Zic1
• UniProt Synonym Gene Names: Zic
• UniProt Entry Name: ZIC1_MOUSE

Uniprot Description

ZIC1: Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally- located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'- TGGGTGGTC-3'. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; nucleic acid binding; DNA binding; metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nervous system development; central nervous system development; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; adult walking behavior; pattern specification process; regulation of transcription, DNA-dependent; positive regulation of protein import into nucleus; spinal cord development; positive regulation of transcription from RNA polymerase II promoter; brain development; cell differentiation; skeletal development; regulation of smoothened signaling pathway