Rabbit BLM Polyclonal Antibody | anti-BLM antibody

BLM polyclonal antibody

Cat. Num. AAA3013774

• Gene Names: BLM; BS; RECQ2; RECQL2; RECQL3
• Reactivity: Human, Mouse, Rat, Pig, Horse, Rabbit
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: BLM; Polyclonal Antibody; BLM polyclonal antibody; Blooms Syndrome Protein Blm; BLM_HUMAN; Bloom Syndrome; Bloom syndrome protein; Bloom syndrome RecQ helicase like; BS; DNA Helicase; DNA helicase RecQ like type 2; MGC126616; MGC131618; MGC131620; RECQ 2; RECQ like; RecQ like type 2; RecQ protein like 3; RecQ Protein-like 3; RECQ-2; RECQ-Like; RecQ-like type 2; RECQ2; RECQL 2; RECQL 3; RECQL-2; RECQL-3; RECQL2; RECQL3; type 2; anti-BLM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Pig, Horse, Rabbit
• Clonality: Polyclonal
• Specificity: BLM polyclonal Antibody detects endogenous levels of BLM protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-BLM antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human BLM:761-860/1417
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BLM ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BLM ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BLM ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BLM ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Related Product Information for anti-BLM antibody

Bloom's syndrome is an autosomal recessive disorder characterized by pre- and post-natal growth deficiencies, sun sensitivity, immunodeficiency and a predisposition to various cancers. The gene responsible for Bloom's syndrome, BLM, encodes a protein homologous to the RecQ helicase of E. coli and is mutated in most Bloom's syndrome patients. One characteristic of Bloom's syndrome is an increased frequency of sister chromatid exchange (SCE). BLM has been shown to unwind G4 DNA, and a failure of this function is thought to be responsible for the increased rate of SCE. BLM is known to be translocated to the nucleus, where its ATPase activity is stimulated by both single- and double-stranded DNA. Mutations in the yeast SGS1, a homolog of BLM, are known to cause mitotic hyperrecombination similiar to that observed in Bloom's cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4557365
• NCBI GeneID: 641
• NCBI Accession #: NP_000048.1
• NCBI GenBank Nucleotide #: NM_000057.2
• UniProt Accession #: P54132; Q52M96
• Molecular Weight: 159,000 Da
• NCBI Official Full Name: Bloom syndrome protein
• NCBI Official Synonym Full Names: Bloom syndrome, RecQ helicase-like
• NCBI Official Symbol: BLM
• NCBI Official Synonym Symbols: BS; RECQ2; RECQL2; RECQL3
• NCBI Protein Information: Bloom syndrome protein; recQ protein-like 3; DNA helicase, RecQ-like type 2
• UniProt Protein Name: Bloom syndrome protein
• Protein Family: Bloom syndrome protein
• UniProt Gene Name: BLM
• UniProt Synonym Gene Names: RECQ2; RECQL3; RecQ2
• UniProt Entry Name: BLM_HUMAN

NCBI Description

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]

Uniprot Description

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: DNA repair, damage; DNA replication; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: male germ cell nucleus; nuclear chromosome; lateral element; chromosome, telomeric region; PML body; nuclear matrix; pronucleus; cytoplasm; nucleolus; replication fork; nucleus

Molecular Function: four-way junction helicase activity; G-quadruplex DNA binding; ATP-dependent DNA helicase activity; protein binding; ATP-dependent 3'-5' DNA helicase activity; p53 binding; ATPase activity; ATP-dependent helicase activity; bubble DNA binding; helicase activity; single-stranded DNA binding; ATP binding

Biological Process: negative regulation of DNA recombination; regulation of binding; positive regulation of transcription, DNA-dependent; negative regulation of mitotic recombination; DNA double-strand break processing; alpha-beta T cell differentiation; negative regulation of cell division; DNA repair; double-strand break repair via homologous recombination; DNA duplex unwinding; protein oligomerization; DNA recombination; replication fork processing; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA strand renaturation; positive regulation of alpha-beta T cell proliferation; replication fork protection; regulation of cyclin-dependent protein kinase activity; response to DNA damage stimulus; telomere maintenance; response to X-ray

Disease: Bloom Syndrome