Western Blot (WB)
(Western blot analysis of KIR2DL5B Antibody (Center) in K562 cell line lysates (35ug/lane). KIR2DL5B (arrow) was detected using the purified Pab.)
Rabbit anti-Human KIR2DL5B Polyclonal Antibody | anti-KIR2DL5B antibody
KIR2DL5B (Killer Cell Immunoglobulin-like Receptor 2DL5B, KIR2DL5, KIR2DL5.2, CD158 Antigen-like Family Member F2, CD158F2, CD158F, Killer Cell Immunoglobulin-like Receptor 2DLX, KIR2DLX) (APC)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
KIR2DL5B; Polyclonal Antibody; KIR2DL5B (Killer Cell Immunoglobulin-like Receptor 2DL5B; KIR2DL5; KIR2DL5.2; CD158 Antigen-like Family Member F2; CD158F2; CD158F; Killer Cell Immunoglobulin-like Receptor 2DLX; KIR2DLX) (APC); Killer Cell Immunoglobulin-like Receptor Two Domains Long Cytoplasmic Tail 5B; anti-KIR2DL5B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KIR2DL5B.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-KIR2DL5B antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
FLISA: 1:1000
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1000
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the center region, between a portion within aa270-299, of human KIR2DL5B, conjugated to KLH.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of KIR2DL5B Antibody (Center) in K562 cell line lysates (35ug/lane). KIR2DL5B (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of KIR2DL5B Antibody (Center) in K562 cell line lysates (35ug/lane). KIR2DL5B (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(KIR2DL5B Antibody (Center) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIR2DL5B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(KIR2DL5B Antibody (Center) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIR2DL5B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(KIR2DL5B Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(KIR2DL5B Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-KIR2DL5B antibody
References
1. Gomez-Lozano N., et.al., Immunogenetics 54:314-319(2002).
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
40,607 Da
NCBI Official Full Name
Killer cell immunoglobulin-like receptor 2DL5B
UniProt Protein Name
Killer cell immunoglobulin-like receptor 2DL5B
Protein Family
UniProt Gene Name
KIR2DL5B
UniProt Synonym Gene Names
CD158F; CD158F2; KIR2DL5; KIR2DLX
UniProt Entry Name
KI2LB_HUMAN
Uniprot Description
KIR2DL5B: Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: plasma membrane; integral to membrane
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Product Notes
The KIR2DL5B kir2dl5b (Catalog #AAA6484416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIR2DL5B (Killer Cell Immunoglobulin-like Receptor 2DL5B, KIR2DL5, KIR2DL5.2, CD158 Antigen-like Family Member F2, CD158F2, CD158F, Killer Cell Immunoglobulin-like Receptor 2DLX, KIR2DLX) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KIR2DL5B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 FLISA: 1:1000 IHC: 1:50-1:100 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KIR2DL5B kir2dl5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIR2DL5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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