ZBTB24 sirna

ZBTB24 siRNA (Mouse)

Cat. Num. AAA8226196

• Gene Names: Zbtb24; Bif1; Bsg1; ZNF450; BC055367
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZBTB24; ZBTB24 siRNA (Mouse); BIF1; BSG1; ZNF450; Zinc finger and BTB domain-containing protein 24; Bone morphogenetic protein-induced factor 1; Brain-specific protein 1; Zinc finger protein 450; ZBTB24 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZBTB24 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 354

• Applicable Applications for ZBTB24 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZBTB24 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZBTB24 sirna

siRNA to inhibit ZBTB24 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 469663638
• NCBI GeneID: 268294
• NCBI Accession #: NP_001264158.1
• NCBI GenBank Nucleotide #: NM_001277229.1
• UniProt Accession #: Q80X44; Q3UIB7; Q7TPC4; Q8CJC9
• Molecular Weight: 40,007 Da
• NCBI Official Full Name: zinc finger and BTB domain-containing protein 24 isoform 2
• NCBI Official Synonym Full Names: zinc finger and BTB domain containing 24
• NCBI Official Symbol: Zbtb24
• NCBI Official Synonym Symbols: Bif1; Bsg1; ZNF450; BC055367
• NCBI Protein Information: zinc finger and BTB domain-containing protein 24
• UniProt Protein Name: Zinc finger and BTB domain-containing protein 24
• Protein Family: Zinc finger and BTB domain-containing protein
• UniProt Gene Name: Zbtb24
• UniProt Synonym Gene Names: Bif1; Bsg1; Znf450
• UniProt Entry Name: ZBT24_MOUSE

NCBI Description

This gene encodes a protein containing eight C2H2-type zinc fingers and a BTB domain. Expression of this gene is induced by bone morphogenetic protein-2 signaling. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies syndrome-2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

ZBTB24: May be involved in BMP2-induced transcription. Defects in ZBTB24 are the cause of immunodeficiency- centromeric instability-facial anomalies syndrome type 2 (ICF2). A rare disorder characterized by a variable immunodeficiency resulting in recurrent infections, facial anomalies, and branching of chromosomes 1, 9, and 16. Other variable symptoms include growth retardation, failure to thrive, and psychomotor retardation. Laboratory studies show limited hypomethylation of DNA in a small fraction of the genome in some, but not all, patients. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Cellular Component: nucleus

Molecular Function: DNA binding; nucleic acid binding; metal ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; hemopoietic progenitor cell differentiation