Mouse anti-Human CREB1 Monoclonal Antibody | anti-CREB1 antibody

CREB1 (Cyclic AMP-responsive Element-binding Protein 1, CREB-1, cAMP-responsive Element-binding Protein 1) APC

Cat. Num. AAA6136043

• Gene Names: CREB1; CREB; CREB-1
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CREB1; Monoclonal Antibody; CREB1 (Cyclic AMP-responsive Element-binding Protein 1; CREB-1; cAMP-responsive Element-binding Protein 1) APC; anti-CREB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F1-1B2
• Specificity: Recognizes human CREB1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CREB1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-341 from human CREB1 (AAH10636) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNHVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (63.25kD).)

Western Blot (WB)

(Western Blot analysis of CREB1 expression in transfected 293T cell line by CREB1 monoclonal antibody. Lane 1: CREB1 transfected lysate (37kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CREB1 on formalin-fixed paraffin-embedded human lymph node tissue. [antibody concentration 1-10ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CREB1 on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged CREB1 is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-CREB1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 14714955
• NCBI GeneID: 1385
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: Homo sapiens cAMP responsive element binding protein 1, mRNA
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB; CREB-1
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• Protein Family: Cyclic AMP-responsive element-binding protein

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]