WNT5A sirna

WNT5A siRNA (Mouse)

Cat. Num. AAA8205664

• Gene Names: Wnt5a; Wnt-5a; 8030457G12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT5A; WNT5A siRNA (Mouse); WNT-5A; Protein Wnt-5a; WNT5A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT5A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 360

• Applicable Applications for WNT5A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT5A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT5A sirna

siRNA to inhibit WNT5A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 371940979
• NCBI GeneID: 22418
• NCBI Accession #: NP_001243153.1
• NCBI GenBank Nucleotide #: NM_001256224.1
• UniProt Accession #: P22725; Q8BM17; Q8BMF9; Q8VCV6
• Molecular Weight: 40,489 Da
• NCBI Official Full Name: protein Wnt-5a isoform 2
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 5A
• NCBI Official Symbol: Wnt5a
• NCBI Official Synonym Symbols: Wnt-5a; 8030457G12Rik
• NCBI Protein Information: protein Wnt-5a
• UniProt Protein Name: Protein Wnt-5a
• Protein Family: Protein
• UniProt Gene Name: Wnt5a
• UniProt Synonym Gene Names: Wnt-5a
• UniProt Entry Name: WNT5A_MOUSE

Uniprot Description

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; extracellular region; intracellular

Molecular Function: protein domain specific binding; protein binding; receptor tyrosine kinase-like orphan receptor binding; frizzled binding; cytokine activity; transcription factor activity; frizzled-2 binding; receptor binding

Biological Process: neural tube development; positive regulation of JNK activity; activation of MAPK activity; multicellular organismal development; positive regulation of transcription, DNA-dependent; embryonic skeletal development; negative chemotaxis; positive regulation of endocytosis; negative regulation of synaptogenesis; positive regulation of interleukin-1 beta secretion; Wnt receptor signaling pathway through beta-catenin; uterus development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of BMP signaling pathway; anterior/posterior pattern formation; positive regulation of fibroblast proliferation; regulation of protein localization; cell-cell signaling; convergent extension; determination of anterior/posterior axis, embryo; positive regulation of mesenchymal cell proliferation; JNK cascade; embryonic limb morphogenesis; positive regulation of macrophage activation; positive regulation of cytokine production; somitogenesis; inner ear morphogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; positive regulation of interleukin-6 production; vagina development; digestive tract morphogenesis; negative regulation of fat cell differentiation; positive regulation of angiogenesis; genitalia development; Wnt receptor signaling pathway, calcium modulating pathway; organ morphogenesis; positive regulation of protein catabolic process; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; axon guidance; wound healing; hypophysis morphogenesis; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of cytokine secretion during immune response; positive regulation of JNK cascade; palate development; negative regulation of axon extension involved in axon guidance; signal transduction; activation of JNK activity; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; morphogenesis of an epithelium; establishment of planar polarity; heart looping; cell differentiation; negative regulation of epithelial cell proliferation; cervix development; cell migration; Wnt receptor signaling pathway; positive regulation of meiosis; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; positive regulation of peptidyl-serine phosphorylation; limb morphogenesis; positive regulation of ossification; positive regulation of cGMP metabolic process; development of primary male sexual characteristics; convergent extension involved in organogenesis; embryonic development; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; cartilage development; ameboidal cell migration; neural tube closure; hindgut morphogenesis; positive regulation of protein amino acid phosphorylation; neurite morphogenesis; lung development; positive regulation of inflammatory response