UCP1 sirna

UCP1 siRNA (Mouse)

Cat. Num. AAA8223984

• Gene Names: Ucp1; Ucp; Slc25a7; AI385626
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UCP1; UCP1 siRNA (Mouse); SLC25A7; UCP; Mitochondrial brown fat uncoupling protein 1; UCP 1; Solute carrier family 25 member 7; Thermogenin; UCP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UCP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 307

• Applicable Applications for UCP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UCP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UCP1 sirna

siRNA to inhibit UCP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678497
• NCBI GeneID: 22227
• NCBI Accession #: NP_033489.1
• NCBI GenBank Nucleotide #: NM_009463.3
• UniProt Accession #: P12242
• Molecular Weight: 33,248 Da
• NCBI Official Full Name: mitochondrial brown fat uncoupling protein 1
• NCBI Official Synonym Full Names: uncoupling protein 1 (mitochondrial, proton carrier)
• NCBI Official Symbol: Ucp1
• NCBI Official Synonym Symbols: Ucp; Slc25a7; AI385626
• NCBI Protein Information: mitochondrial brown fat uncoupling protein 1
• UniProt Protein Name: Mitochondrial brown fat uncoupling protein 1
• Protein Family: Mitochondrial brown fat uncoupling protein
• UniProt Gene Name: Ucp1
• UniProt Synonym Gene Names: Slc25a7; Ucp; UCP 1
• UniProt Entry Name: UCP1_MOUSE

Uniprot Description

UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: mitochondrial envelope; membrane; mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane

Molecular Function: protein binding; oxidative phosphorylation uncoupler activity

Biological Process: regulation of transcription from RNA polymerase II promoter; proton transport; transport; transmembrane transport; mitochondrial transport