TIE1 sirna

TIE1 siRNA (Mouse)

Cat. Num. AAA8232621

• Gene Names: Tie1; TIE; tie-1; D430008P04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TIE1; TIE1 siRNA (Mouse); TIE; TIE-1; Tyrosine-protein kinase receptor Tie-1; TIE1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TIE1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1134

• Applicable Applications for TIE1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TIE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TIE1 sirna

siRNA to inhibit TIE1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 144446133
• NCBI GeneID: 21846
• NCBI Accession #: NP_035717.2
• NCBI GenBank Nucleotide #: NM_011587.2
• UniProt Accession #: Q06806; Q811F4; Q8BGI2
• Molecular Weight: 124,583 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• NCBI Official Symbol: Tie1
• NCBI Official Synonym Symbols: TIE; tie-1; D430008P04Rik
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: Tie1
• UniProt Synonym Gene Names: Tie; Tie-1
• UniProt Entry Name: TIE1_MOUSE

Uniprot Description

TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Tie family

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: blood vessel development; negative regulation of angiogenesis; response to retinoic acid; in utero embryonic development; angiogenesis; vasculogenesis; protein amino acid phosphorylation; negative regulation of cell migration; phosphorylation; plasma membrane fusion