TGFBR1 sirna

TGFBR1 siRNA (Mouse)

Cat. Num. AAA8200407

• Gene Names: Tgfbr1; ALK5; ESK2; Alk-5; TGFR-1; TbetaRI; AU017191; TbetaR-I
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TGFBR1; TGFBR1 siRNA (Mouse); TGF-beta receptor type-1; TGFR-1; ESK2; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I; TGFBR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TGFBR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 503

• Applicable Applications for TGFBR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TGFBR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TGFBR1 sirna

siRNA to inhibit TGFBR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678323
• NCBI GeneID: 21812
• NCBI Accession #: NP_033396.1
• NCBI GenBank Nucleotide #: NM_009370.3
• UniProt Accession #: Q64729; A2AJN0
• Molecular Weight: 55,790 Da
• NCBI Official Full Name: TGF-beta receptor type-1 isoform 1
• NCBI Official Synonym Full Names: transforming growth factor, beta receptor I
• NCBI Official Symbol: Tgfbr1
• NCBI Official Synonym Symbols: ALK5; ESK2; Alk-5; TGFR-1; TbetaRI; AU017191; TbetaR-I
• NCBI Protein Information: TGF-beta receptor type-1
• UniProt Protein Name: TGF-beta receptor type-1
• Protein Family: TGF-beta receptor
• UniProt Gene Name: Tgfbr1
• UniProt Synonym Gene Names: TGFR-1; TGF-beta receptor type I; TbetaR-I
• UniProt Entry Name: TGFR1_MOUSE

NCBI Description

This gene encodes a member of the transforming growth factor beta (TGF-beta) receptor family of proteins. These proteins comprise one component of the TGF-beta signaling pathway, which transduces extracellular signals into gene expression changes to regulate a wide range of cellular responses, including proliferation, migration, differentiation and apoptosis. Homozygous knockout mice for this gene exhibit impaired angiogenesis and embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

TGFBR1: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Found in all tissues; most abundant in placenta and least abundant in brain and heart.

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; EC 2.7.11.30; Receptor, misc.; TKL group; STKR family; Type1 subfamily

Cellular Component: protein complex; tight junction; membrane; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; caveola; cell junction; endosome; receptor complex; lipid raft

Molecular Function: metal ion binding; punt binding; nucleotide binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; transferase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; transforming growth factor beta binding; protein heterodimerization activity; transforming growth factor beta receptor activity, type I; ubiquitin protein ligase binding; growth factor binding; transferase activity, transferring phosphorus-containing groups; protein complex binding; kinase activity; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: blastocyst development; collagen fibril organization; activation of MAPKK activity; heart development; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; cell motility involved in cell locomotion; protein amino acid phosphorylation; post-embryonic development; anterior/posterior pattern formation; regulation of growth; transforming growth factor beta receptor signaling pathway; germ cell migration; transmembrane receptor protein serine/threonine kinase signaling pathway; kidney development; neuron fate commitment; embryonic cranial skeleton morphogenesis; parathyroid gland development; positive regulation of cell growth; response to estrogen stimulus; regulation of gene expression; negative regulation of endothelial cell proliferation; endothelial cell migration; negative regulation of apoptosis; lens development in camera-type eye; apoptosis; regulation of protein ubiquitination; protein amino acid autophosphorylation; palate development; signal transduction; regulation of transcription, DNA-dependent; negative regulation of endothelial cell differentiation; positive regulation of cell proliferation; angiogenesis; cell differentiation; skeletal development; positive regulation of filopodium formation; pharyngeal system development; thymus development; in utero embryonic development; male gonad development; peptidyl-threonine phosphorylation; positive regulation of cell motility; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; embryonic development; skeletal morphogenesis; regulation of protein binding; epithelial to mesenchymal transition; artery morphogenesis; phosphorylation