T sirna

T siRNA (Mouse)

Cat. Num. AAA8200833

• Gene Names: T; Lr; T1; Bra; Low; Tl2; Tl3; cou; me75; D17Mit170
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: T; T siRNA (Mouse); Brachyury protein; Protein T; T sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: T siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 436

• Applicable Applications for T sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse T gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for T sirna

siRNA to inhibit T expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6678203
• NCBI GeneID: 20997
• NCBI Accession #: NP_033335.1
• NCBI GenBank Nucleotide #: NM_009309.2
• UniProt Accession #: P20293
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: brachyury protein
• NCBI Official Synonym Full Names: brachyury
• NCBI Official Symbol: T
• NCBI Official Synonym Symbols: Lr; T1; Bra; Low; Tl2; Tl3; cou; me75; D17Mit170
• NCBI Protein Information: brachyury protein
• UniProt Protein Name: Brachyury protein
• Protein Family: THP3
• UniProt Gene Name: T
• UniProt Entry Name: BRAC_MOUSE

Uniprot Description

Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.

Protein type: DNA-binding; Cell cycle regulation; Motility/polarity/chemotaxis; Transcription factor

Cellular Component: cytoplasm; nucleus; chromatin

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; somitogenesis; transcription, DNA-dependent; neural plate morphogenesis; embryonic skeletal development; multicellular organismal development; mesoderm migration; notochord formation; reproductive process; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; notochord development; cardiac muscle cell differentiation; anterior/posterior pattern formation; BMP signaling pathway; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; neural tube closure; penetration of zona pellucida; positive regulation of transcription from RNA polymerase II promoter; mesoderm development; vasculogenesis

Disease: Neural Tube Defects