Mouse Brachyury Protein (T) ELISA Kit | T elisa kit

Mouse Brachyury Protein (T) ELISA Kit

Cat. Num. AAA9716926

• Gene Names: T; Lr; T1; Bra; Low; Tl2; Tl3; cou; Tbxt; me75; D17Mit170
• Reactivity: Mouse
• Synonyms: Brachyury Protein (T); Mouse Brachyury Protein (T) ELISA Kit; T; RP11-459F1.1; MGC104817; TFT; OTTHUMP00000017588; T brachyury homolog; T brachyury-like; transcription factor T; T elisa kit

• Reactivity: Mouse
• Specificity: Mouse Brachyury protein (T) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse T. No significant cross-reactivity or interference between Mouse T and analogues was observed.
• Sequence Length: 436

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Brachyury protein (T) Standard Curve.)

Related Product Information for T elisa kit

Description: This Mouse Brachyury protein (T) ELISA Kit employs a two-site sandwich ELISA to quantitate T in samples. An antibody specific for T has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any T present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Brachyury appears to have a conserved role in defining the midline of a bilaterian organism,and thus the establishment of the anterior-posterior axis; this function is apparent in chordates and molluscs. Its ancestral role, or at least the role it plays in the Cnidaria, appears to be in defining the blastopore. It also defines the mesoderm during gastrulation.Tissue-culture based techniques have demonstrated one of its roles may be in controlling the velocity of cells as they leave the primitive streak. Transcription of genes required for mesoderm formation and cellular differentiation. Brachyury has also been shown to help establish the cervical vertebral blueprint during fetal development. The number of cervical vertebrae is highly conserved among all mammals; however a spontaneous vertebral and spinal dysplasia (VSD) mutation in this gene has been associated with the development of six or fewer cervical vertebrae instead of the usual seven.

NCBI and Uniprot Product Information

• NCBI GI #: 6678203
• NCBI GeneID: 20997
• NCBI Accession #: NP_033335.1
• NCBI GenBank Nucleotide #: NP_033335.1
• UniProt Accession #: P20293
• NCBI Official Full Name: T-box transcription factor T
• NCBI Official Synonym Full Names: brachyury, T-box transcription factor T
• NCBI Official Symbol: T
• NCBI Official Synonym Symbols: Lr; T1; Bra; Low; Tl2; Tl3; cou; Tbxt; me75; D17Mit170
• NCBI Protein Information: T-box transcription factor T; brachyury protein
• UniProt Protein Name: Brachyury protein
• UniProt Gene Name: T

Uniprot Description

Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.

Protein type: Cell cycle regulation; DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 17 A1|17 4.92 cM

Cellular Component: chromatin; cytoplasm; nuclear chromatin; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; protein binding; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

Biological Process: anatomical structure morphogenesis; anterior/posterior pattern specification; BMP signaling pathway; cardiac muscle cell differentiation; embryonic skeletal system development; heart morphogenesis; mesoderm development; mesoderm migration involved in gastrulation; multicellular organism development; negative regulation of transcription from RNA polymerase II promoter; neural plate morphogenesis; neural tube closure; notochord development; notochord formation; penetration of zona pellucida; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; reproductive process; somitogenesis; transcription, DNA-dependent; vasculogenesis; Wnt receptor signaling pathway through beta-catenin

Disease: Neural Tube Defects